Sequences from confirmation amplicon


Confirmation sequences for line 454D08 and hit At2g17640 are shown.
Line ID 454D08
Locus At2g17640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7670264
Primers used for PCR 20vh - 8474, show primer details
   
Sequence Name 55-K117914-0022-454-D08C-20vh
GenBank Accession LN508930 [GenBank]
Sequencing primer gene specific primer, 20vh: 5'-TTTGGTTGGATTAAATATCAAGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K117914-0022-454-D08C-20vh
ATTCTAACCTATGGATACTACTATAACACTGTGGATTACATGGAGACGCATTACCATGAC
ATTTACTTTATCTGAACAAGAATAAGAATCGATGAAGCAGAGAAAGTGTTAGAATTTGAG
GGAAACCTCAAGTTTAGCTTCTTCTCTTATAGCATCCCAAATAGGATCAGAACGAGGATC
AAGCAAATTCGAGTCAAACTCTGACTTATGAGTTCCCTTAGCGTAAACCTCGAAACCACT
CTCGAAAGGAAGCTCATCGCCATTCATATCTCTCCAAGGAGACGATAAAGAAGAATCACA
AACTCCGTTCAGCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGC
   
Sequence Name 15-K117913-0022-454-D08C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K117913-0022-454-D08C-8409
GTATGTTGGTCAATATGGAGAAAAAGAAAGAGTAATTACCTATTTTTTTTCAATTCAGAA
ATGTACCTCTCCGCACCATT

Last Updated on 06.12.2018 16:11