Sequences from confirmation amplicon


Confirmation sequences for line 454F05 and hit At3g54490 are shown.
Line ID 454F05
Locus At3g54490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20175245
Primers used for PCR BB60 - 8474, show primer details
   
Sequence Name 31-K104153-0022-454-F05C-BB60
GenBank Accession LN500140 [GenBank]
Sequencing primer gene specific primer, BB60: 5'-TGGTATATGTTTTGGAAAAGCTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K104153-0022-454-F05C-BB60
ATTTTTTCTTGGGACCAGAAATTTGTTGTAGCAGTAAGGTTCCTCGCATGGTACTTGTGT
TGTAACGAATGGCATATTGCTTTAACTTTATAATGGGAAAGGATGGGATCATATTAAGGT
GTCATTAAAATGTTATGTTTGAACAGCTAGTTGAGATGAGAATGTAACCAGACATATTCT
CATGCTTTTACCTGTTTTTCATTAAGCTTATTGAAATGGTTTTATGCAGATCCTTGTTGT
GTTCATGGGAACTGAACCAATCACCGTGAAGTCAGTTCGTGCTCTTCATATTCAGATTTC
CAACAATGTAGGCTTGCATGCTATGATTCTGGTTTTACAGAGCAAAATGAACCACTTTGC
CCAGAAGGCATTGACTACCTTCCCTTTTACAGTTGAAACTTTCCCGGTCAGCACTTATCT
GTTTTCCTACATGATGAAACATTATAGCTCTGGATTTGGATTGTCTTGCATGATATCTAT
NGAGGATCATGAAATTGAGACCTGAATTGGAAATACGTTAACTGAACAGTTACATGTTTA
TACTTATTGAATATGCCAACAGATTGAAGATTTGTTGGTCAACATCACAAAGCACATTCA
GCAGCCAAAGATTGAGATTTTAAACAAAGAGGAGAAAGAACAACTGCTGAGGAAGCATGC
ACTTGAGGACAAACAGGTCTGTTTATATTCTGCACAAAGCTTCTTTGCCTATNTGTGTAT
GCTATGTTTTAATGGATGTTTCTTTTCCATTTCAGCTTCCTTATTTGCAGGAGAAGGACA
GCTTTGTGAGGTACTATGGGTTAAAGAAAAAACAGGTGGTGAAGATTACCGATGCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAA
   
Sequence Name 31-K104154-0022-454-F05C-8409
GenBank Accession LN500141 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K104154-0022-454-F05C-8409
TTCGGACATGCATCGGTAATCTTCACCACCTGTTTTTTCTTTAACCCATAGTACCTCACA
AAGCTGTCCTTCTCCTGCAAATAAGGAAGCTGAAATGGAAAAGAAACATCCATTAAAACA
TAGCATACACAAATAGGCAAAGAAGCTTTGTGCAGAATATAAACAGACCTGTTTGTCCTC
AAGTGCATGCTTCCTCAGCAGTTGTTCTTTCTCCTCTTTGTTTAAAATCTCAATCTTTGG
CTGCTGAATGTGCTTTGTGATGTTGACCAACAAATCTTCAATCTGTTGGCATATTCAATA
AGTATAAACATGTAACTGTTCAGTTAACGTATTTCCAATTCAGGTCTCAATTTCATGATC
CTCAATAGATATCATGCAAGACAATCCAAATCCAGAGCTATAATGTTTCATCATGTAGGA
AAACAGATAAGTGCTGACCGGGAAAGTTTCAACTGTAAAAGGGAAGGTAGTCAATGCCTT
CTGGGCAAAGTGGTTCATTTTGCTCTGTAAAACCAGAATCATAGCATGCAAGCCTACATT
GTTGGAAATCTGAATATGAAGAGCACGAACTGACTTCACGGTGATTGGTTCAGTTCCCAT
GAACACAACAAGGATCTGCATAAAACCATTTCAATAAGCTTAATGAAAAACAGGTAAAAG
CATGAGAATATGTCTGGTTACATTCTCATCTCAACTAGCTGTTCAAACATAACATTTTAA
TGACACCTTAATATGATCCCATCCTTTCCCATTATAAAGTTAAAGCAATATGCCATTCGT
TACAACACAAGTACCATGCGAGGAACCTTACTGCTACNACAAATTTTCTTGGTTCCCAAG
AAAAAAATGGGTATGTATTAGTTCTTCAGCTTT

Last Updated on 06.12.2018 16:11