Sequences from confirmation amplicon


Confirmation sequences for line 454F09 and hit At5g65060 are shown.
Line ID 454F09
Locus At5g65060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25991243
Primers used for PCR KF26 - 8474, show primer details
   
Sequence Name 81-K103173-0022-454-F09C-8409
GenBank Accession LN514056 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K103173-0022-454-F09C-8409
TTCTTTCGCNAAAAGAAAAGAGCTTCGTTTTGTTTTACCTTTATTTCCACATTGGCGCGA
GGAAGATAAAAAGGTTGAGATTATTATGCAGCTTTTTATAATTTACAATTTTGTAAAAGC
TTCTGAATCAGGCTGTGAGTAAGTTTATGGTGAAAGCTCAGCCGTTGATGATGGTGATTA
CTTGAGCAGCGAAAGAGTCTCCGGTACTTTGTTGCCGGAGCCATTTTCCCGTGACATTCC
TCTGTCACCTTCTATAACCAGAAACGTCTTCTTCCCCACCTGTAAGAAACTTTCTTACAT
TATTTTCCTACAGCTAACAAAAATTAACAAGAAATCTAGATTTTTAAGAGTATACTTTTC
TTTTGCTTAAAACTAAATTGTGAGTTTCACTCANAACTGTGTTGCA
   
Sequence Name 82-K103173-0022-454-F09C-KF26
GenBank Accession LN514180 [GenBank]
Sequencing primer gene specific primer, KF26: 5'-ATGCAACACAGTTTTGAGTGAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K103173-0022-454-F09C-KF26
CAAGAAAGTATACTCTTAAAATCTAGATTTCTTGTTAATTTTTGTTAGCTGTAGGAAAAT
AATGTAAGAAAGTTTCTTACAGGTGGGGAAGAAGACGTTTCTGGTTATAGAAGGTGACAG
AGGAATGTCACGGGAAAATGGCTCCGGCAACAAAGTACCGGAGACTCTTTCGCTGCTCAA
GTAATCACCATCATCAACGGCTGAGCTTTCACCATAAACTTACTCACAGCCTGATTCAGA
AGCTTTTACAAAATTGTAAATTATAAAAAGCTGCATAATAATCTCAACCTTTTTATCTTC
CTCGCGCCCATGTGGAAATAAAGGTAAAACAAAACGAAGCTCTTTTCTTTTATGCGAAAG
AATTGTAAAACTAAGATNATGAGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
TTCCGCAGCGTTNTNATAAAATGAAAGTACATTTTGATAAAACGACCAATTACGATCCGT
CGTATTTATNGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTNTATNTCGACG
TGTCTACNTTCACGTCCAAATGGGGGCTTAGATGAGAAACTTCACGATNGATGCCTTGAT
NTCGCCNTTC

Last Updated on 06.12.2018 16:11