Sequences from confirmation amplicon


Confirmation sequences for line 454H06 and hit At1g61800 are shown.
Line ID 454H06
Locus At1g61800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22825958
Primers used for PCR RK64 - 8474, show primer details
   
Sequence Name 42-K020068-022-454-H06C-8409
GenBank Accession LN504303 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K020068-022-454-H06C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATAAAAACCAAATCAATAATCTTT
AATTGTTTGTTAAGTTTTATAGACTCCAACTCAAATGACTATGAGTGGATTGGTATATAT
TGCTTTAGTTCCCTTCTAACTTAAGATATAAGATTCTAACTTAATGTGTTGTTTTTGTTT
TTTGAATCAATAGGTGGGTAGTGGCACAAAGTGTGTTTTACCATTTGTACAATCAGGTCT
CATACATGTCATTAGACCAGATTTCGCCGTTAACTTTCAGTATCGGTAATACGATGAAGC
GGATTTCCGTTATTGTTGCATCGATCATCATTTTCCATACCCCGATTCAACCGGTTAATG
CCCTCGGTGCTGCCATTGCGATTTTTGGAACTTTCCTCTACTCACAGGTAAACAAACAAA
CTTATTCTTGTTTACCTACCTCTAGTACTTGGTTCTTTAGTGAACAATTTCTAGTTCTAA
TATAATTCATTGCGTTCTCCTATGTTCTTTGCAATCATTGAGCAGGCGAAGCAGTGAGGA
TGGTTTAGGAGGCAGTTTTTTGGGTTTGTTTTGCAAAAGAAAATGAAAACTAACAAAAGA
GTAGCCGG
   
Sequence Name 41-K020068-022-454-H06C-RK64
GenBank Accession LN503914 [GenBank]
Sequencing primer gene specific primer, RK64: 5'-GGTCTGATCAAGAAATGACACTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K020068-022-454-H06C-RK64
TATCTTTATTATTACGATCATCTAAATAAAACATAGATAATATAGAATTAGCCTGTCTTG
TGCAGAATCTATTATGAGACAATGAAAAAAAAAGTAAAAGCAAACACTCTATCAACTTCA
AGCTCCAGTTTCTTCATATCCATGGACCGGGGAAAAGCATTTTCCGATGTTGATCTCACC
GGAATGTTCTCTCCTCCCCGGCTACTCTTTTGTTAGTTTTCATTTTCTTTTGCAAAACAA
ACCCAAAAAACTGCCTCCTAAACCATCCTCACTGCTTCGCCTGCTCAATGATTGCAAAGA
ACATAGGAAGAACGTCAATGAATTATATTAGAACTAGAAATTGTTCACTAAAGAACCAAG
TACTAGAGGTAGGTAAACAAGAATAAGTTTGTTTGTTTACCTGTGAGTAGAGGAAAGTTC
CAAAAATCGCAATGGCAGCACCGAGGGCATTAACCGGTTGAATCGGGGTATGGAAAATGA
TGATCGATGCAACAATAACGGAAATCCGCTTCTCGTATTACCGATACTGAAAGTTAACGG
CGAAATCTGGTCTAATGACATGTATGAGACCTGATTGTACAAATGGTAAAACACACTTTG
TGCCACTACCCACCTATTGATTCAAAAAACAAAAACAACACATTAAGTTAGAATCTTATA
TCTTAAGTTAGAAGGGAACTAAAGCAAT

Last Updated on 06.12.2018 16:11