Sequences from confirmation amplicon


Confirmation sequences for line 455F06 and hit At3g20290 are shown.
Line ID 455F06
Locus At3g20290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7078369
Primers used for PCR 17vd - 8474, show primer details
   
Sequence Name 20-K117753-0022-455-F06C-17vd
GenBank Accession LN495395 [GenBank]
Sequencing primer gene specific primer, 17vd: 5'-GTTCTGGGGCATATGTGTAAGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K117753-0022-455-F06C-17vd
TTCTTTTCCTCTCCCTTGCCGTCGAGACGAAGAACCCGCTTTCTCTGAAACCGTAATCTC
ACGTATACATCAACCAACTTTTTTTTTTTTTTTTTTTTTTTGAAAAGGGGGGGTTTTAAA
CCCCCCTTTTTTTCCCCCCCAAAAAATTTAAAATTGGGGAAACCCCAAAATGGGGTTCCC
CCCCAAAAAATTTTTTAAACGGGACCCCCCCTAATTTTTTCCACCGGAAAAGGGAAAAAA
AACCCCCCCCCGGGGGTTTTTTTCCCGGGGAAAAAAAAAAAACCCAAAGGGGGGGGGTAA
AATCCCCCAATTGGGGGCCTTTTTAGGGTTTTTTTTGGGGTTGGGGGTTTTCAACCTCCA
AATTCCCCTCCCCAAAAAGGGGCAAAAAAATATTTCCCTTTTGGGGGGAAAAAAAAAAAA
GGGGAAGGCCCTTTTTTGGGGAAAAAAGGCCAAAAATTTTCCCCAAATTAAAAATTCCCC
CCCCCCGAAATAAAAACGGGAAAGGTTTTTGGTGGGGGGAAATTTTTCAATTTAAAAAGG
GCCCCCGGCCGGGAAAACCCCCGGGGAACCCCAGGGGTTAGGGGGGCCAATGGGGAAAAA
AAAAAAAAATTTTCCCCTTTTTTTTTTCATTCCAAAAAGGAAA
   
Sequence Name 04-K117752-0022-455-F06C-8409
GenBank Accession LN488093 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K117752-0022-455-F06C-8409
GCCATTTACATTGAAGAACTTGCCTCAACACAAAATCCTTACCTGCTTCAATTCCGGACG
AGGCAAATTCGACATAGTGAAGAACTTGATCGCATCGTTACCAGTAATACGGCCATCGCC
GTCTGCTCATTTACACCAAACAGAGAATAAGCTTTTAGCGCATATAGTGAAGACGAGAAT
CGAAGACTGAAGAACACAAGACAACAAAATGAACATGAAACGAGACCTGAATCGGAGAAT
TCGAACCATTCCTTGTAGATCATTTGATTCTCCTTGGAACAAGAACCAGCTGCGACGGAT
TCGATCTCCATCTCCGATTGCAAAAATCTAGAGATGTACCGATTAACAAACTTCTCAGGT
GTGAATCCAGATTTGTGGATTCGCAGATATTGAACTTATACGGAGAAGAACAAAAGGTAA
GCTAAACAACAACAATTACNAAAAAAAAAAAAAAAAAAAAAGGGGGGGGGGGGTTAACCG
GAAAATTGGGGTTTAAAAAAAGGGGGTTTTTTTTTCCCCGGGGGGGGGGGGGAAAAAAAA
ATTTCCCCGGGGAAAAATTTCCCTTTTTTTTTAAAAATTAAAAAAATGCCCCCAAAAAAA
A

Last Updated on 06.12.2018 16:11