Sequences from confirmation amplicon


Confirmation sequences for line 455G12 and hit At2g17560 are shown.
Line ID 455G12
Locus At2g17560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7643264
Primers used for PCR NA31 - 8474, show primer details
   
Sequence Name 41-K101564-0022-455-G12C-8409
GenBank Accession LN504021 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K101564-0022-455-G12C-8409
TTTCTCGTCTTCCTGTAATTTCACGAATCTTCCTCCGATTTTCAAATTTTCGAATTGATT
TTTCGATTCTAATCAAATTTTAAACTGATTGTTGTTGGATCTTAGTGAGGATTTCCGAAA
AGAGTTTAACCTAGCGAATCCTAATAACAAATCCGTCGCTACTGTGAGTATATATTTCTC
CGAAATTTCTGGAAAAATTTCTCAGATCTGTAATCTGAAAACTGGTTTAGGTTGGTTTAA
TTATCGGATTGGTTTGTCTAATCAGGTTGGTAAGGCTGCTGGAGCTAGATGGAAGGCAAT
GACTGATGAAGTAAGTCATAATAATAGTAGCCATTGAAATGCTTGATTTGGGGAATAAGG
TTTTTGTAGATCTGTATTTAAAACTTTGTGCTTGTTTGAATCTGTAATTACAGGATAAAG
CTCCTTACGTCGCTNAGGCTGAGAGCAGAAAGACTGAATATATTAAGAATGTGCAACAGT
ACAACTTGAAACTGGTATAAACTCTCACACTGTATAGAGAGATTGTTCTTTTGGGTCTTC
TTCTTATGCTG
   
Sequence Name 41-K101577-0022-455-G12C-NA31
GenBank Accession LN504022 [GenBank]
Sequencing primer gene specific primer, NA31: 5'-ACAGCATAAGAAGAAGACCCAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K101577-0022-455-G12C-NA31
ACCAGTTTCAGTTGTACTGTTGCACATTCTTAATATATTCAGTCTTTCTGCTCTCAGCCT
TAGCGACGTAAGGAGCTTTATCCTGTAATTACAGATTCAAACAAGCACAAAGTTTTAAAT
ACAGATCTACAAAAACCTTATTCCCCAAATCAAGCATTTCAATGGCTACTATTATTATGA
CTTACTTCATCAGTCATTGCCTTCCATCTAGCTCCAGCAGCCTTACCAACCTGATTAGAC
AAACCAATCCGATAATTAAACCAACCTAAACCAGTTTTCAGATTACAGATCTGAGAAATT
TTTCCAGAAATTTCGGAGAAATATATACTCACAGTAGCGACGGATTTGTTATTAGGATTC
GCTAGGTTAAACTCTTTTCGGAAATCCTCACTAAGATCCAACAACAATCAGTTTAAAATT
TGATTAGAATCGAAAAATCAATTCGAAAATTTGAAAATCGGAGGAAGATTCGTGAAATTA
CAGGAAGACGAAGAAAGCACTTGGAGGTCAATATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATATCCG

Last Updated on 06.12.2018 16:11