Sequences from confirmation amplicon


Confirmation sequences for line 456B06 and hit At3g27960 are shown.
Line ID 456B06
Locus At3g27960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10380723
Primers used for PCR NC01 - 8474, show primer details
   
Sequence Name 17-K026966-022-456-B06C-8409
GenBank Accession LN493708 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K026966-022-456-B06C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGCTTCAATGTGTTAGA
TGATGCTATAGAGATATTGGAGTATGTTGTTGGGACAAGAGAAGAGAAGCTCGGGACGGC
AAATCCCGAGGTTGAGGACGAGAAGCAGAGGCTTGCTGCGTTGTTGAAAGAAGCTGGAAG
AGGAAGGAGTAAAAGAAACAGAGCACTTCTTACCCTTTTGGACAACAACCCTGAAATTGC
AAATGGACAGAGACCGGTTTATTGATTAACCGGATTATTGTCCGGTCTAAAATGTATATA
TATACAAAATACAAGTTCGAATACGGTACTTACACGGTCGAGGGAGAAGGAAAAAAATGA
AGAGGCTTTATGTTTTCTAGTTTTATAAAAAGTGAAGGAAAAAAAAAAAAAATCTGGCAT
TTGTTTTCTTTATAAATAAAATGTAAAGAGTAAAACGCATTTGATTTGGCCGTTTTGAAA
GGGGATTTACATTGAAATTTTGGAGTTTAAAAATCTCCT
   
Sequence Name 18-K026966-022-456-B06C-NC01
GenBank Accession LN494155 [GenBank]
Sequencing primer gene specific primer, NC01: 5'-TAGAGAAACTGCCATCAAGGAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K026966-022-456-B06C-NC01
TTTTAAACTCCAAAATTTCAATGTAAATCACATTACAAAACGACCAAATCAATTGCGTTT
TACTCTTTACATTTTATTTATAAAGAAAACAAATGCAAGATTTTTTTTTTTTTCCCTTCC
CTTTTTAAAAAACTAGAAAACAAAAAGCCCCTTCATTTTTTTCCTTCCCCCCCGACGGGG
AAAGTCCCGAATTCAAACTGGTATTTGGAAAATATAAACATTTAAAACCGGAAAAAAACC
CGGTAAATCAAAAAACGGGTCTCGGCCCATTGGCAATTTCGGGGTGGTTGCCCAAAAGGG
AAAAAAGGGCCCGGTTTCTTTAACCCCTTCCTCTTCCAGCTTCTTTAAAAAACGAAGAAG
GCCTCGGCTTCTCGCCCTCAACCTGGGGATTGGCCGCCCCGAGTTTCTCTTCTCTGGTCC
CAACAACAAACCCCAATATCTCTATGGCACCATCTAACACATGGAAGCGGGATATATTCA
ATGGAAAAGGGCTCCAGGCCCGGGAAATCTCCTGGGATCACCAAGGAGTATGAGGGCCAA
TAGGGAGAAAAAGAAAGAGTAATTCCCAATTTTTTTTCAATTCAAAAAGGTAAATGCCCG
CGGCGTTTTTATAAAAGGAAGGTCCTTTTGGATAAAACGACAAAT

Last Updated on 06.12.2018 16:11