Sequences from confirmation amplicon


Confirmation sequences for line 456F02 and hit At5g48760 are shown.
Line ID 456F02
Locus At5g48760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19772676
Primers used for PCR JH13 - 8474, show primer details
   
Sequence Name 66-K101271-0022-456-F02C-8409
GenBank Accession LN511559 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K101271-0022-456-F02C-8409
TTCGGACTGAGCCTTTACATTGAATATATCCTGTGATCAGGGATATGCTCGAAGCGCGTT
GTCGTCGACGCGCGCCACCACATGCTTGGCCGTCTTGCGTCGATCACCGCTAAAGAGCTT
CTCAATGGCCAGAAAGTTGTCATTGTCCGGTGCGAAGAGATTTGCCTCTCCGGCGGACTC
GTTCGTCAGAAAATGAAGTACATGAGGTTTCTTCGCAAACGTATGAACACTAAACCTTCT
CATGGACCTATTCACTTCCGTGCTCCTTCCAAAATCTTCTGGCGTACTGTTCGCGGGTTC
GTATATCAATCTCTCTTATCTCTCCAATTAGATGAGGTTTCGAGAAATTGATGGTTCTTG
CTCTGTATGGATTAAGTTTGTGTTTGATACTTATGAGTGAATCATTTGTGGTTGATTTCA
ATTCAATGCTTTTGAAATGATGATTTAGATAGCAGAGTGATTCATAGTTGTTATGTGTTT
TGATTTGTGAAGTATGATTCCACACAAGACGAAGCGTGGAGCTGCTGCATTAGCACGTCT
GAAGGTATATGAAGGTGTTCCTACTCCATATGACAAGATCAAGAGGATGGTCATCCCTGA
TGCTCTCAAGTTTGTTCCTTTTACCATTTTTGTTCTTCTCTTTTGGATCCGTAACTG
   
Sequence Name 66-K101277-0022-456-F02C-JH13
GenBank Accession LN511560 [GenBank]
Sequencing primer gene specific primer, JH13: 5'-AACAGTTACGGATCCAAAAGAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K101277-0022-456-F02C-JH13
ACTTGAGAGCATCAGGGATGACCATCCTCTTGATCTTGTCATATGGAGTAGGAACACCTT
CATATACCTTCAGACGTGCTAATGCAGCAGCTCCACGCTTCGTCTTGTGTGGAATCATAC
TTCACAAATCAAAACACATAACAACTATGAATCACTCTGCTATCTAAATCATCATTTCAA
AAGCATTGAATTGAAATCAACCACAAATGATTCACTCATAAGTATCAAACACAAACTTAA
TCCATACAGAGCAAGAACCATCAATTTCTCGAAACCTCATCTAATTGGAGAGATAAGAGA
GATTGATATACGAACCCGCGAACAGTACGCCAGAAGATTTTGGAAGGAGCACGGAAGTGA
ATAGGTCCATGAGAAGGTTTAGTGTTCATACGTTTGCGAAGAAACCTCATGTACTTCATT
TTCTGACGAACGAGTCCGCCGGAGAGGCAAATCTCTTCGCACCGGACAATGACAACTTTC
TGGCCATTGAGAAGCTCTTTAGCGGTGATCGACGCANGACGGCCAAGCATGTGGTGGCGC
GCGTCGACAACNACGCGCTTCGAGCATATCCCTGATCACAGGATATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11