Sequences from confirmation amplicon


Confirmation sequences for line 456H04 and hit At5g47190 are shown.
Line ID 456H04
Locus At5g47190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19165288
Primers used for PCR 08uf - 8474, show primer details
   
Sequence Name 21-K117188-0022-456-H04C-08uf
GenBank Accession LN495833 [GenBank]
Sequencing primer gene specific primer, 08uf: 5'-ACTGTAGTTGTTTTGGCTCCTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K117188-0022-456-H04C-08uf
TGAATGTTGATTTGCTTTTTGTTTCTGTAGCTACTGAACAAGAAAGCTATTGAGGTTGCA
GAGACTGTAAGACCGGTTCCTGGACTTAGGACTGGGGATATTGTAGAAATCAAACTGGTA
AAACTCATTGTCCCCGTATAATCTTATGCAATTTATGCATTTAACTGCAAGATTTTGATA
CGACATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCA
   
Sequence Name 06-K117187-0022-456-H04C-8409
GenBank Accession LN488987 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K117187-0022-456-H04C-8409
ATTTACATGTCGTATCAAAATCTTGCAGTTAAATGCATAAATTGCATAAGATTATACGGG
GACAATGAGTTTTACCAGTTTGATTTCTACAATATCCCCAGTCCTAAGTCCAGGAACCGG
TCTTACAGTCTCTGCAACCTCAATAGCTTTCTTGTTCAGTAGCTACAGAAACAAAAAGCA
AATCAACATTTCATTTCTCCAATGCCTAAAAGATAACTGATGATTTAAGAACAGGAGCCA
AAACAACTACAGT

Last Updated on 06.12.2018 16:11