Sequences from confirmation amplicon


Confirmation sequences for line 458B03 and hit At4g37640 are shown.
Line ID 458B03
Locus At4g37640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17685178
Primers used for PCR 40gp - 8474, show primer details
   
Sequence Name 43-K112250-0022-458-B03C-40gp
GenBank Accession LN504885 [GenBank]
Sequencing primer gene specific primer, 40gp: 5'-TTCTCTGTGGTGATAGATGAATCG-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K112250-0022-458-B03C-40gp
GTGATGCCAGAATCCTTTCCTTATGTCTGGAACCAAGTGCAAGATGGATCTTGTAAGATG
ATGATTACTACAGTTGGGATGAGGACTCAATGGGGAAAGCTAATGGCGACTTTGACCGAA
GGAGGGGATGATGAAACTCCACTGCAGGTGAAACTCAATGGAGTTGCTACCATCATTGGC
AAAATTGGTCTTTTCTTTGCGGTGGTTACTTTCGCGGTTCTGGTGCAAGGAATGTTTATG
AGGAAGCTTTCAACGGGAACTCACTGGGTCTGGTCTGGTGATGAAGCATTGGAGCTCTTG
GAATACTTTGCTATTGCTGTGACAATCGTTGTGGTTGCGGTTCCTGAAGGATTGCCTTTA
GCTGTGACTCTAAGTCTTGCGTTTGCCATGAAGAAAATGATGAACGATAAAGCTCTTGTG
AGGCATTTAGCAGCATGTGAGACTATGGGATCTGCAACGACGATNTGCAGTGACAAGACT
GGTACACTCACAACCAATCACATGACCGTTGTGAAGTCTTGCATTTGCATGATGGAATCC
CGGAATCCCGGAATTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATTCCG
   
Sequence Name 33-K112249-0022-458-B03C-8409
GenBank Accession LN501053 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K112249-0022-458-B03C-8409
ATCGGGATTCATCATGCAATGCAGACTTCACAACGGTCATGTGATTGGTTGTGAGTGTAC
CAGTCTTGTCACTGCAAATCGTCGTTGCAGATCCCATAGTCTCACATGCTGCTAAATGCC
TCACAAGAGCTTTATCGTTCATCATTTTCTTCATGGCAAACGCAAGACTTAGAGTCACAG
CTAAAGGCAATCCTTCAGGAACCGCAACCACAACGATTGTCACAGCAATAGCAAAGTATT
CCAAGAGCTCCAATGCTTCATCACCAGACCAGACCCAGTGAGTTCCCGTTGAAAGCTTCC
TCATAAACATTCCTTGCACCAGAACCGCGAAAGTAACCACCGCAAAGAAAAGACCAATTT
TGCCAATGATGGTAGCAACTCCATTGAGTTTCACCTGCAGTGGAGTTTCATCATCCCCTC
CTTCGGTCAAAGTCGCCATTAGCTTTCCCCATTGAGTCCTCATCCCAACTGTAGTAATCA
TCATCTTACAAGATCCATCTTGCACTTTGGTTCCAGACATAAGGAAAGGATTCTGCGCAT
TCACCATCACAGGCTCACTTTCACCAGTCAAACTCGATTCATCTATA

Last Updated on 06.12.2018 16:11