Sequences from confirmation amplicon


Confirmation sequences for line 458D07 and hit At3g50310 are shown.
Line ID 458D07
Locus At3g50310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18648844
Primers used for PCR 43gp - 8474, show primer details
   
Sequence Name 80-K111813-0022-458-D07C-8409
GenBank Accession LN513964 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K111813-0022-458-D07C-8409
TTTACGGCGNTTAGAAAAAGTGTGGAGATTAGAGGGACGCCGNTTATACATGGCACCGGA
ATGTGTTAATGATAACGAGTATGGATCAGCGGCTGACGTGTGGGCTTTAGGATGTGCTGT
AGTTGAGATGTTTAGTGGCAAAACGGCATGGAGTGTTAAAGAAGGATCACACTTCATGTC
GCTTTTGATACGTATAGGTGTTGGTGATGAGTTACCGAAGATTCCAGAGATGTTGTCCGA
AGAAGGCAAGGATTTTTTGTCGAAGTGTTTTGTTAAAGATCCAGCGAAAAGATGGACGGC
TGAGATGCTTTTGAACCATTCGTTTGTAACCATTGATCTTGAAGATGATCACCGAGAAAA
CTTCGTTGTGAAGGTTAAGGATGAGGATAAAGTATTGATGTCTCCAAAGTGTCCTTTTGA
GTTTGAGAATTGGGATT
   
Sequence Name 88-K111814-0022-458-D07C-43gp
GenBank Accession LN514879 [GenBank]
Sequencing primer gene specific primer, 43gp: 5'-AATCCCAATCATCAAACTCAAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K111814-0022-458-D07C-43gp
TCCTTACCTTCACAACGAAGTTTTCTCGGTGATCATCTTCAAGATCAATGGTTACAAACG
AATGGTTCAAAAGCATCTCAGCCGTCCATCTTTTCGCTGGATCTTTAACAAAACACTTCG
ACAAAAAATCCTTGCCTTCTTCGGACAACATCTCTGGAATCTTCGGTAACTCATCACCAA
CACCTATACGTATCAAAAGCGACATGAAGTGTGATCCTTCTTTAACACTCCATGCCGTTT
TGCCACTAAACATCTCAACTACAGCACATCCTAAAGCCCACACGTCAGCCGCTGATCCAT
ACTCGTTATCATTAACACATTCCGGTGCCATGTATAACGGCGTCCCTCTAATCTCCACAC
TTTTTCTTAACGCCGTTAAATCTCCGTCAACTCTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTC

Last Updated on 06.12.2018 16:11