Sequences from confirmation amplicon


Confirmation sequences for line 458E04 and hit At1g31290 are shown.
Line ID 458E04
Locus At1g31290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11189579
Primers used for PCR BE38 - 8474, show primer details
   
Sequence Name 93-K111634-0022-458-E04C-BE38
GenBank Accession LN515259 [GenBank]
Sequencing primer gene specific primer, BE38: 5'-CGTAAGCCTCAACTTTTCTCTGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>93-K111634-0022-458-E04C-BE38
CTACGTCGTTGCTTCTCACACCGCTGTGGTAAGCACCATAACTCTAGATTTTTCTTTGCC
ATATCTTTAAGCATTTGCTATTTTTCTAACGTTCAAGTAACTAAACAATGAGTTTGTTTT
AAGGTGATTAATACAGAATGAGTTTGAGAAAAACTTATGTTCTACATTACTACCTTGGAG
AATTAAAATTACCCAAAATTGCTTAAATCAATAATTTTAGAATTGCTTACAAGAAATAAT
TTTAGAACTTAACTAATTCTGAATATTATAAGAATATTAAACATAAACAAAGATATATTA
TATGAATACCTTTTTTGGTTTAATGAATATTTATGTGTTTTTGAGCTACAGAGATTAACA
AATAAGCAATGAGTATGATGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 85-K111633-0022-458-E04C-8409
GenBank Accession LN514563 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K111633-0022-458-E04C-8409
ATTTACATTGAATAATCCATCATACTCATTGCTTATTTGTTAATCTCTGTAGCTCAAAAA
CACATAAATATTCATTAAACCAAAAAAGGTATTCATATAATATATCTTTGTTTATGTTTA
ATATTCTTATAATATTCAGAATTAGTTAAGTTCTAAAATTATTTCTTGTAAGCAATTCTA
AAATTATTGATTTAAGCAATTTTGGGTAATTTTAATTCTCCAAGGTAGTAATGTAGAACA
TAAGTTTTTCTCAAACTCATTCTGTATTAATCACCTTAAAACAAACTCATTGTTTAGTTA
CTTGAACGTTAGAAAAATAGCAAATGCTTAAAGATATGGCAAAGAAAAATCTAGAGTTAT
GGTGCTTACCACTAGCGGTGTGAGAAGCAACGACGTTAGACGAAGAACTAGAAGGTAAGA
CCGTCGAATCAGAGAAAAGTGAGGGCTTACG

Last Updated on 06.12.2018 16:11