Sequences from confirmation amplicon


Confirmation sequences for line 458F09 and hit At4g32480 are shown.
Line ID 458F09
Locus At4g32480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15676445
Primers used for PCR 18vd - 8474, show primer details
   
Sequence Name 21-K117753-0022-458-F09C-18vd
GenBank Accession LN495840 [GenBank]
Sequencing primer gene specific primer, 18vd: 5'-AGCATCGAGTATATCCGAATCTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K117753-0022-458-F09C-18vd
CAGCTGTCTTAGCCATCAGATCCGACTTAGTTCCCAATCATATACTGACACGTCATCTTC
TTCCAAACTCAAAACAGAGCATCGAAGCACTCTTTCGCGGCGCCTTGTATCTGTTTTTTC
TCCCACCTCTCAGGCGGGTCCCTAACCCCTTATCCGGGTCTTACTTAAGCCTTCAATTTT
TCTGACTATATATATCCCTTCCACTTATTTCTATTTTCTTCATCGAAACAAAAAATAATT
AAGAGAGAAAAAAACAAAAAACATTTTCCACTCATGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 05-K117752-0022-458-F09C-8409
GenBank Accession LN488535 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K117752-0022-458-F09C-8409
GTTTTTTGTTTTTTTCTCTCTTAATTATTTTTTGTTTCGATGAAGAAAATAGAAATAAGT
GGAAGGGATATATATAGTCAGAAAAATTGAAGGCTTAAGTAAGACCCGGATAAGGGGTTA
GGGACCCGCCTGAGAGGTGGGAGAAAAAACAGATACAAGGCGCCGCGAAAGAGTGCTTCG
ATGCTCTGTTTTGAGTTTGGAAGAAGATGACGTGTCAGTATATGATTGGGAACTAAGTCG
GATCTGATGGCTAAAGACAGCTAGATCTTATGTCAGCTTGTCCCAACTTCGGACCACAGA
TTCGGATTACTTCGATGCT

Last Updated on 06.12.2018 16:11