Sequences from confirmation amplicon


Confirmation sequences for line 459C10 and hit At5g58950 are shown.
Line ID 459C10
Locus At5g58950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23802146
Primers used for PCR 13kn - 8474, show primer details
   
Sequence Name 61-K113845-0022-459-C10C-13kn
GenBank Accession LN510575 [GenBank]
Sequencing primer gene specific primer, 13kn: 5'-CCATTAGACAGTTCTTGTGGCATA-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K113845-0022-459-C10C-13kn
ACAGAATATTTTACCTTATGTACGACTGCGAAAGCAGCTTGAATAGGATTCATGTCCTCA
TATGGGATTGCTCCAGCTACCATTTCCCATAAAACGAGTCCAAAGCTGTATACATCGGCC
TTACGTCCATGTGGTTTCCGTTTTATCATTTCAGGTGCCATCCACCTATATGTTCCGGGG
TCATCAGCCAACATGTCACAGTACTCCTCCTCGCACGCTATGCCAAAGTCAGCGATCTTC
AAATGGAATTCTTCGTCGATCAATACATTTTCTGGCTTAAGATCCCGATGAATTATGCGT
CGTGAGTGAATATATTCCATCCCTCTAGCAATATCTATAGCAAACTCTATAAGCTTTTTC
AAAGGAAGAGACCTATTCTCAGGCTTGTGCAGAAAAGATCTTAAAGATCCTTCAGGTAAA
TACTGCGTGAGGACACAATATACAGGCGGATCTTTGTACGCTCCAACAAACTGTTTTTAG
CACATAAATAAGAGAAGAGAAGTTAACAAAAACCGTAATTACCAATTTTTTTGCACCCGA
AGGCCGGAAACACTGCCGCTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGC
   
Sequence Name 34-K113844-0022-459-C10C-8409
GenBank Accession LN501490 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K113844-0022-459-C10C-8409
CATTTCAGCGGCAGTGTTTCCGGCCTTCGGGTGCAAAAAAATTGGTAATTACGGTTTTTG
TTAACTTCTCTTCTCTTATTTATGTGCTAAAAACAGTTTGTTGGAGCGTACAAAGATCCG
CCTGTATATTGTGTCCTCACGCAGTATTTACCTGAAGGATCTTTAAGATCTTTTCTGCAC
AAGCCTGAGAATAGGTCTCTTCCTTTGAAAAAGCTTATAGAGTTTGCTATAGATATTGCT
AGAGGGATGGAATATATTCACTCACGACGCATAATTCATCGGGATCTTAAGCCAGAAAAT
GTATTGATCGACGAAGAATTCCATTTGAAGATCGCTGACTTTGGCATAGCGTGCGAGGAG
GAGTACTGTGACATGTTGGCTGATGACCCCGGAACATATAGGTGGATGGCACCTGAAATG
ATAAAACGGAAACCACATGGACGTAAGGCCGATGTATACAGCTTTGGACTCGTTTTATGG
GAAATGGTAGCTGGAGCAATCCCATATGAGGACATGAATCCTATTCAAGCTGCTTTCGCA
GTCGTACATAAGGTAAAATATTCTGTGCGTCTCCTTACTATTTCATTATTGTAATCTTCC
GTAGAGTTTATGCCACAAGACTGNTCCTAATGG

Last Updated on 06.12.2018 16:11