Sequences from confirmation amplicon


Confirmation sequences for line 459G11 and hit At2g24710 are shown.
Line ID 459G11
Locus At2g24710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10517303
Primers used for PCR 38nb - 8474, show primer details
   
Sequence Name 57-K114597-0022-459-G11C-38nb
GenBank Accession LN509492 [GenBank]
Sequencing primer gene specific primer, 38nb: 5'-ATGGTACTTTCTCGTTCTCGTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K114597-0022-459-G11C-38nb
CACAGAATTAAATCCACAATAACCAGCATGAGTAGTTTGCTTGAAAAAGGAGAAACAGTG
GGTTATCAGCGAACATCCTTCATCCTTGGAAAGCTTAAAGAAAGAGGTTTCCCGCAATCT
AGCCTTGTGCCCTTTGATACTGCAGAAGAATGTGATGAGCTTCTAAGTAAAGGACCAAAA
AAAGGTGGTGTCTCTGGAGCTTTCTTGGAAATACCTTACTTGAGACTCTTTCTTGGACAG
TTTTGCAACACATACAAAATGGTTGAAGAACCCTTCAATGTTGATGGATTCGGCTTTGTA
AGTTCCTATATCAAAACTTATGTGAAAATTGCATTAAATGGTGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCG
   
Sequence Name 17-K114596-0022-459-G11C-8409
GenBank Accession LN493954 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K114596-0022-459-G11C-8409
ATTTTCACATAAGTTTCGATATAGGAACTTACAAAGCCGAATCCATCAACATTGAAGGGT
TCTTCAACCATTTTGTATGTGTTGCAAAACTGTCCAAGAAAGAGTCTCAAGTAAGGTATT
TCCAAGAAAGCTCCAGAGACACCACCTTTTTTTGGTCCTTTACTTAGAAGCTCATCACAT
TCTTCTGCAGTATCAAAGGGCACAAGGCTAGATTGCGGGAAACCTCTTTCTTTAAGCTTT
CCAAGGATGAAGGATGTTCGCTGATAACCCACTGTTTCTCCTTTTTCAAGCAAACTACTC
ATGCTGGTTATTGTTGGATTTAATTTCTGTGATGTCAAAAGCGATGCCAAACTCGCTGTG
TAACTCTGGGTTAACACGAGAACG

Last Updated on 06.12.2018 16:11