Sequences from confirmation amplicon


Confirmation sequences for line 461C12 and hit At5g50020 are shown.
Line ID 461C12
Locus At5g50020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20352357
Primers used for PCR 14kn - 8474, show primer details
   
Sequence Name 80-K113669-0022-461-C12C-14kn
GenBank Accession LN513973 [GenBank]
Sequencing primer gene specific primer, 14kn: 5'-ACATTGGTGAGCTTTATTGGATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K113669-0022-461-C12C-14kn
CTTAATTTCGATCTTTTTCACTCTATCACTGTTGATAGTGTCATTTATTGTTATCATTGC
GAAGATCTAAGTTTGAACATATATACCGTATTTGTTAGATTTGGTTTGGTTCCATGCTGA
TGCATCACCATATTTACAACATTGATGAGCTTTCTATTCACTATTGGTTCACTCTCCAAG
CATGAACTTGCATTTTTGGTGAACTCTATCAGTCTATTGTCAGATTTTGTTCAATGGAAC
CCATTACCACTTGTGAATGTAGCTTTTGATGCAATGCTCTATGCTGTATTTATTCTGAAC
ATGATGATCATCTTCTCTTTTGTTGGTCTTTTGCAGAAGTTTCTGTTTGGTGGGAGATTG
ATATTTGGGCCAGATGCTTGGTCTATTCCTTTTACCTTCCTGCTCATCATAACTCCCGTT
TGTTTCTTACATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCACGNTTATT
   
Sequence Name 49-K113666-0022-461-C12C-8409
GenBank Accession LN506909 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K113666-0022-461-C12C-8409
ATGTAAGAAACAACGGGAGTTATGATGAGCAGGAAGGTAAAAGGAATAGACCAAGCATCT
GGCCCAAATATCAATCTCCCACCAAACAGAAACTTCTGCAAAAGACCAACAAAAGAGAAG
ATGATCATCATGTTCAGAATAAATACAGCATAGAGCATTGCATCAAAAGCTACATTCACA
AGTGGTAATGGGTTCCATTGAACAAAATCTGACAATAGACTGATAGAGTTCACCAAAAAT
GCAAGTTCATGCTTGGAGAGTGAACCAATAGTGAATAGAAAGCTCATCAATGTTGTAAAT
ATGGTGATGCATCAGCATGGAACCAAACCAAATCTAACAAATACGGTATATATGTTCAAA
CTTAGATCTTCGCAATGATAACAATAAATGACACTATCAACAGTGATAGAGTTGAAAAAG
ATTCGAAATTTAAAGCCACCAAATTCAACATATACAGTCGAATCCAATAAAGCTCACCAA
TG

Last Updated on 06.12.2018 16:11