Sequences from confirmation amplicon


Confirmation sequences for line 461D01 and hit At1g07530 are shown.
Line ID 461D01
Locus At1g07530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2315451
Primers used for PCR TA26 - 8474, show primer details
   
Sequence Name 11-K030219-022-461-D01C-8409
GenBank Accession LN490988 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K030219-022-461-D01C-8409
ATTGAATATAGATGATGAATTGGCTATGCAGTTCAAGAAAGGGGTTGAGGAAGCTAGTAA
GTTCCTTCCTAAGTCTTCTCAGCTCTTTATTGATGTGGATAGTTACATCCCTATGAATTC
TGGTTCCAAGGAAAATGGTTCTGAGGTTTTTGTTAAGACGGAGAAGAAAGATGAGACAGA
GCATCATCATCATCATAGCTATGCTCCTCCTCCCAACAGATTAACTGGTAAGAAAAGCCA
TTGGCGCGACGAAGATGAAGATTTCGTTGAAGAAAGAAGTAACAAGCAATCAGCTGTTTA
TGTTGAGGAAAGCGAGCTTTCTGAAATGTTTGATAAGATCTTGGTATGTGGCCCTGGGAA
ACCTGTATGCATTCTTAACCAGAACTTTCCTACAGAATCCGCTAAAGTCGTGACCGCACA
GTCAAATGGAGCAAAGATTCGTGGGAAGAAATCAACTTCTACTAGTCATAGTAACGATTC
TAAGAAAGAAACTGCTGATTTGAGGACTCTTTTGGTGTTATGTGCACAAGCTGTATCAGT
   
Sequence Name 12-K030219-022-461-D01C-TA26
GenBank Accession LN491448 [GenBank]
Sequencing primer gene specific primer, TA26: 5'-TCTACGATCATCCACTGATACAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K030219-022-461-D01C-TA26
TCTCAATCAGCAGTTTCTTTCTTAGAATCGTTACTATGACTAGTAGAAGTTGATTTCTTC
CCACGAATCTTTGCTCCATTTGACTGTGCGGTCACGACTTTAGCGGATTCTGTAGGAAAG
TTCTGGTTAAGAATGCATACAGGTTTCCCAGGGCCACATACCAAGATCTTATCAAACATT
TCAGAAAGCTCGCTTTCCTCAACATAAACAGCTGATTGCTTGTTACTTCTTTCTTCAACG
AAATCTTCATCTTCGTCGCGCCAATGGCTTTTCTTACCAGTTAATCTGTTGGGAGGAGGA
GCATAGCTATGATGATGATGATGCTCTGTCTCATCTTTCTTCTCCGTCTTAACAAAAACC
TCAGAACCATTTTCCTTGGAACCAGAATTCATAGGGATGTAACTATCCACATCAATAAAG
AGCTGAGAAGACTTAGGAAGGAACTTACTAGCTTCCTCAACCCCTTTCTTGAACTGCATA
GCCAATTCATCATCTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAAC
GACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGG
AAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11