Sequences from confirmation amplicon


Confirmation sequences for line 461D06 and hit At2g15440 are shown.
Line ID 461D06
Locus At2g15440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:6744787
Primers used for PCR 09jk - 8474, show primer details
   
Sequence Name 80-K113193-0022-461-D06C-09jk
GenBank Accession LN513968 [GenBank]
Sequencing primer gene specific primer, 09jk: 5'-CCTTGGAAGTTGATAGATCTCCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K113193-0022-461-D06C-09jk
ATTAGAGGTTCCAGCTGGGCCATGAGAGTGAATGATGTTGGAGATGGTGGAGCGTTCAAG
AAAGGACATAGATGTGTTTGGTGGTGATGAGGAAGTGTAGTGGAGAAGTGCGGCCAGGAC
TGGTGGTGGAAGAGAGGAGGA
   
Sequence Name 43-K113192-0022-461-D06C-8409
GenBank Accession LN504899 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K113192-0022-461-D06C-8409
CATTTACAATTGAATAAAATGAAGAGCAACACAAACACTAATCTGATTCTCTTCCACCAC
CACTCTCCACTAACTTCAAAATCACCCTCCGCCGTCGCCGCCGCGAATCACCACCGTTTC
TTATTCATCTTCTTCCTCTCTTTCTTCACTCTCCTCTTCTCTTTCTCTCTCTTCTCCTCC
TCAATCCACTCCACCACATCCTCTCACTCTCTCTCCTCATCTCCCTCCTCCTCCTCTCTT
CCACCACCAGTCCTCGCCGCACTTCTCCACTACACTTCCTCATCACCACCAAACACATCT
ATGTCCTTTCTTGAACTCTCCACCATCTCCAACATCATTCACTCTCATGGCCCAGCTTGT
AACCTCTTAATCTTTGGACTAACTCACGAATCTCTTCTCTGGAGATCTATCAACTTCCAA
GG

Last Updated on 06.12.2018 16:11