Sequences from confirmation amplicon


Confirmation sequences for line 462B04 and hit At1g19780 are shown.
Line ID 462B04
Locus At1g19780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6835025
Primers used for PCR IL29 - 8474, show primer details
   
Sequence Name 56-K032419-022-462-B04C-8409
GenBank Accession LN509007 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K032419-022-462-B04C-8409
CGGATGAGCTTTGCAATCTCATGGCTATTTGAATTGGTAAGTACATATCCAGGAGAAGTT
TTGTTTTCAATTGCAATTGCTGTAGCTGGACTTCTTTTGTTTGCTCTTCTCATTGGGAAT
ATGCAAACTTATCTTCAGTCACTTACGGTTCGCTTAGAGGAAATGAGGATTAAAAGACGT
GATTCCGAACAGTGGATGCATCACAGGTCACTTCCACAAAACCTGAGGGAACGAGTCAGA
CGTTATGATCAATACAAATGGTTGGAAACAAGAGGAGTCGACGAAGAAAACATAGTCCAG
AGTTTACCAAAAGATCTTAGAAGAGACATCAAACGCCATCTCTGTCTAAATTTAGTTCGC
AGGGTAAGTTTTCAGAAATGTGAATTGCCTAAAGATCCTACACTTCATTCACAAGGAACA
ATGTTCTTTCTTTTTTTTGCAGGTTCCTCTGTTTGCTAATATGGATGAGAGATTACTAGA
CGCAATATGTGAGAGACTAAAGCCAAGTCTATACACAGAGAGCACTACNTTAGTGCGA
   
Sequence Name 56-K032419-022-462-B04C-IL29
GenBank Accession LN509008 [GenBank]
Sequencing primer gene specific primer, IL29: 5'-TCGCACTATGTAAGTGCTCTCTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K032419-022-462-B04C-IL29
ATTGCGTCAGNAATCTCTCATCCATATTAGCAAACAGAGGAACCTGCAAAAAAAAGAAAG
AACATTGTTCCTTGTGAATGAAGTGTAGGATCTTTAGGCAATTCACATTTCTGAAAACTT
ACCCTGCGAACTAAATTTAGACAGAGATGGCGTTTGATGTCTCTTCTAAGATCTTTTGGT
AAACTCTGGACTATGTTTTCTTCGTCGACTCCTCTTGTTTCCAACCATTTGTATTGATCA
TAACGTCTGACTCGTTCCCTCAGGTTTTGTGGAAGTGACCTGTGATGCATCCACTGTTCG
GAATCACGTCTTTTAATCCTCATTTCCTCTAAGCGAACCGTAAGTGACTGAAGATAAGTT
TGCATATTCCCAATGAGAAGAGCAAACAAAAGAAGTCCAGCTACAGCAATTGCAATTGAA
AACAAAACTTCTCCTGGATATGTACTTACCAATTGCAAATAGCCATGTAGATTTGCAATA
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
ATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGA
AAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA
Primers used for PCR FN34 - 3144, show primer details
   
Sequence Name 59-K032734-022-462-B04C-FN34
GenBank Accession LN509906 [GenBank]
Sequencing primer gene specific primer, FN34: 5'-TGTTCAGATTCTTTATTGTGGCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K032734-022-462-B04C-FN34
ATCAGTCCCTGATCTTTTAAGAACAATTGTTCTGCTAAAAGCGATGAGTCTAAATTCAAC
TACGGGATCTATAGCCAGGCTGTGTCTTCCGGTATTGTATCTTCGACAACGTTTTTCTCC
AAGTTTTGTTATTGTCTATGGTGGGGTCTCCAAAATCTCAGGTACCTTCCTTGGAAAATC
GATGAATAGCTGTTAGACTGTGGTGCAATTTTATTGACTTGGGTGTTTTCTTGTATAGCA
CGTTAGGTCAAGGGCTGCAAACAAGTACATATCTTTGCAATTGCAAATCTACATGGCTAT
TGCAATACAATACGACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCC
GGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAA
GGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGC
TTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGG
   
Sequence Name 27-K032734-022-462-B04C-3144
GenBank Accession LN498274 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K032734-022-462-B04C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTCGTAT
TGTATTGCAATAGCCATGTAGATTTGCAATTGCAAAGATATGTACTTGTTTGCAGCCCTT
GACCTAACGTGCTATACAAGAAAACACCCAAGTCAATAAAATTGCACCACAGTCTAACAG
CTATTCATCGATTTTCCAAGGAAGGTACCTGAGATTTTGGAGACCCCACCATAGACAATA
ACAAAACTTGGAGAAAAACGTTGTCGAAGATACAATACCGGAAGACACAGCCTGGCTATA
GATCCCGTAGTTGAATTTAGACTCATCGCTTTTAGCAGAACAATTGTTCTTAAAAAGATC
AGGGACTGATTTGATCCAGTCGGTTTCGCGACTGCTCATTAATTTGCTGCCACAATAA

Last Updated on 06.12.2018 16:11