Sequences from confirmation amplicon


Confirmation sequences for line 462G01 and hit At1g46480 are shown.
Line ID 462G01
Locus At1g46480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17237819
Primers used for PCR IH16 - 8474, show primer details
   
Sequence Name 52-K033546-022-462-G01C-8409
GenBank Accession LN507725 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K033546-022-462-G01C-8409
CCATTTACAATTGAATATTTCATTCTTCTTCCTCCGGCGTCACTTCAGCCACTTTTGACC
TTAAAAACTTCATTAGACCCGATCAAACCGGTCCGACAAAATTTGAACACAAACGAGACC
CTCCTCATCAAGTCAGTTCCATTATTGTACTTCGTACATTTTACATCGTTTCTTTCGTTT
GAAAAAAGAAGAAGAATTCACTCTAGAAGAATTTTGATAATTGCGGCATACCTAATACTT
TGACATTTATAAATTATAACGACTGGAGTGGTTCCTTTTATTATTAATTTTATTTATTTA
TTTTAATGTATGATTCCTAACAAATGTAATGAAATAATGTGTGGGTTCAGTTGGAGACGC
ACCCGGGAGGGACAAGGTGGAACCCGACTCAAGAACAGATAGGGATACTTGAGATGTTGT
ACAAAGGTGGAATGCGTACTCCTAATGCTCAACAGATTGAGCATATCACATTGCAACTCG
GTAAGTACGGGAAAATCGAAGGGAAAAATGTGTTCTATTGTTCCCCAGAA
   
Sequence Name 52-K033546-022-462-G01C-IH16
GenBank Accession LN507726 [GenBank]
Sequencing primer gene specific primer, IH16: 5'-GGTTCTGGAACCAATAGAACACAT-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K033546-022-462-G01C-IH16
CATGTGATATGCTCAATCTGTTGAGCATTAGGAGTACGCATTCCACCTTTGTACAACATC
TCAAGTATCCCTATCTGTTCTTGAGTCGGGTTCCACCTTGTCCCTCCCGGGTGCGTCTCC
AACTGAACCCACACATTATTTCATTACATTTGTTAGGAATCATACATTAAAATAAATAAA
TAAAATTAATAATAAAAGGAACCACTCCAGTCGTTATAATTTATAAATGTCAAAGTATTA
GGTATGCCGCAATTATCAAAATTCTTCTAGAGTGAATTCTTCTTCTTTTTTCAAACGAAA
GAAACGATGTAAAATGTACGAAGTACAATAATGGAACTGACTTGATGAGGAGGGTCTCGT
TTGTGTTCAAATTTTGTCGGACCGGTTTGATCGGGTCTAATGAAGTTTTTAAGGTCAAAA
GTGGCTGAAGTGACGCCGGAGGAAGAAGAATGAAATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGA
AAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAA
ACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACTCCCAAATGG

Last Updated on 06.12.2018 16:11