Sequences from confirmation amplicon


Confirmation sequences for line 463B01 and hit At1g64255 are shown.
Line ID 463B01
Locus At1g64255 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23847001
Primers used for PCR KN51 - 8474, show primer details
   
Sequence Name 06-K103729-0022-463-B01C-KN51
GenBank Accession LN488778 [GenBank]
Sequencing primer gene specific primer, KN51: 5'-AAAGCCTATCCAACAATCTGCTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K103729-0022-463-B01C-KN51
TTTTTAGAATGGATCCTTCATAGTATACGCCATCAGCACCATACTTAATATAACCATTCA
AGTGACACAACACCATAACCTTTTCTCTGACTGTTTCAATCGCCATGAGCACAACAAATC
TGCAGTGAATTCAAAGAAAAAATGTAAAAAATTTAGGCATAGCTTCCTAAAATGAAAAAC
CCTATAAAGCTCAATATCAACGGTAATGTACAGCTCTAAAAGGAGAAAGCTTTCTAGCTA
TAGAAGCAGATAAACTCATTCAAAAACATAAACCCTAAACAGTGATACTCAAAACTAAGA
CTTGTAAAGTTTTACGCAACTGAAAAGAAGAAAAACAGGGAATTTGGAAATGGGTGTCGC
AGAAAAAAGCTTAGATCTTTCAGAAATGGAAGTAATTTCACCTTCGCTAACGATTTCGCC
GGTGATGTTACTGTTACAGGTCAGGTCACTGATGAAGACAACTACTACAGTCTACAGGGA
AATGAGAAATATAGCTTTTCGAAATTTTATAACAGAAAAAAATATAAAAAAATCAATTGG
GCCGACGGAAGAAAGCCCGTGAATATTTGTAGCTTGTTTTACAACTGAAACCAGATTAAT
CCTACTTCCCTAAAGTCATAAGATGCCCGGCTCGGTGCAAACTCAAATCCATCTTTCAGC
TCTTCTTCTTAGTAGCGAGAGGAGTAGTTCTGCATTTGGTACCTGATACGTAAGTTGGAG
GTGATGGAGGTGGTGACGGTGGAATGACAGGAGGAAGCAATCTCGGAACTCCAGAAGCTT
CCGGCCAAGCTGACATTTCCGGAACATGAGAGAAAATGGTGGCGTAAGTTCTTTTTAACC
GTTCAAGAGTGTAACAGTCGTGGGAATTTCAACATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTC
   
Sequence Name 22-K103730-0022-463-B01C-8409
GenBank Accession LN496081 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K103730-0022-463-B01C-8409
GTTGAAATTCCCACGACTGTTACACTCTTGAACGGTTAAAAAGAACTTACGCCACCATTT
TCTCTCATGTTCCGGAAATGTCAGCTTGGCCGGAAGCTTCTGGAGTTCCGAGATTGCTTC
CTCCTGTCATTCCACCGTCACCACCTCCATCACCTCCAACTTACGTATCAGGTACCAAAT
GCAGAACTACTCCTCTCGCTACTAAGAAGAAGAGCTGAAAGATGGATTTGAGTTTGCACC
GAGCCGGGCATCTTATGACTTTAGGGAAGTAGGATTAATCTGGTTTCAGTTGTAAAACAA
GCTACAAATATTCACGGGCTTTCTTCCGTCGGCCCAATTGATTTTTTTATATTTTTTTCT
GTTATAAAATTTCGAAAAGCTATATTTCTCATTTCCCTGTAGACTGTAGTAGTTGTCTTC
ATCAGTGACCTGACCTGTAACAGTAACATCACCGGCGAAATCGTTAGCGAAGGTGAAATT
ACTTCCATTTCTGAAAGATCTAAGCTTTTTTCTGCGACACCCATTTCCAAATTCCCTGTT
TTTCTTCTTTTCAGTTGCGTAAAACTTTACAAGTCTTAGTTTTGAGTATCACTGTTTAGG
GTTTATGTTTTTGAATGAGTTTATCTGCTTCTATAGCTAGAAAGCTTTCTCCTTTTAGAG
CTGTACATTACCGTTGATATTGAGCTTTATAGGGTTTTTCATTTTAGGAAGCTATGCCTA
AATTTTTTACATTTTTTCTTTGAATTCACTGCAGATTTGTTGTGCTCATGGCGATNGAAA
CAGTCAGAGAAAAGGTTATGGTGTTGTGTCACTTGAATGGTTATATTAAGTATGGTGCTG
ATGGCGTATACNATGAAGGATCCATTCTTAAAAAGATCACAATTATGCGAAAGACTGCCT
T

Last Updated on 06.12.2018 16:11