Sequences from confirmation amplicon


Confirmation sequences for line 463C08 and hit At1g24280 are shown.
Line ID 463C08
Locus At1g24280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8610111
Primers used for PCR SD22 - 8474, show primer details
   
Sequence Name 18-K103492-0022-463-C08C-SD22
GenBank Accession LN494269 [GenBank]
Sequencing primer gene specific primer, SD22: 5'-ATTGGGTTTTCTTCTTTATGCCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K103492-0022-463-C08C-SD22
AGGATTGAATCCTGTAGTTCAGCTTGGTTTTAGATAGATTTTCTTATAGAAAGTATCCGA
CTTTAGACAAAACATTGCATGTAATGAATGTTCTCTAATGCGCTTACAATTGTGTGTTTA
GGATCAGTAGCCACCGAGTCAAGCTCGTCCGAGGAGGCGAAAGATGTAGGGGTGTTAACG
ATTCCTTCACTTGAAGCTGACAAGGTTGTAGCAGAGTCTGATGGCGGTGAACAACTTTCA
ACAGTTAGTATCACGGTGGTGGGAGCTTCTGGTGATCTTGCCAAGAAGAAAATATTCCCA
GCTCTTTTCGCACTTTACTATGAAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 42-K103493-0022-463-C08C-8409
GenBank Accession LN504433 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K103493-0022-463-C08C-8409
ATTTACATTGAATAATCCTTCATAGTAAAGTGCGAAAAGAGCTGGGAATATTTTCTTCTT
GGCAAGATCACCAGAAGCTCCCACCACCGTGATACTAACTGTTGAAAGTTGTTCACCGCC
ATCAGACTCTGCTACAACCTTGTCAGCTTCAAGTGAAGGAATCGTTAACACCCCTACATC
TTTCGCCTCCTCGGACGAGCTTGACTCGGTGGCTACTGATCCTAAACACACAATTGTAAG
CGCATTAGAGAACATTCATTACATGCAATGTTTTGTCTAAAGTCGGATACTTTCTATAAG
AAAATCTATCTAAAACCAAGCTGAACTACAGGATTCAATCCTATATATACAAGCTTTCAA
GTTACCGGCAAAGCAAGGCATAAAGAAAANAACCCA

Last Updated on 06.12.2018 16:11