Sequences from confirmation amplicon


Confirmation sequences for line 464F01 and hit At3g48360 are shown.
Line ID 464F01
Locus At3g48360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17909933
Primers used for PCR 05hq - 8474, show primer details
   
Sequence Name 57-K113595-0022-464-F01C-05hq
GenBank Accession LN509479 [GenBank]
Sequencing primer gene specific primer, 05hq: 5'-CGGAACAAAAACTAAACCTAGCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K113595-0022-464-F01C-05hq
CAGAGATGCTGTGTTTTCAAACATATAAGTAAGTTTACCGATTCGGCGTCATCGATGAAC
TGGAGAATGTCGAGCTCGAGGAAAGGATCATGTTCTTGAATAAACTTCCATCCTTCAGTC
TGCTCAACGGTCTTAAACTGCGAATGGATCAGACGCATAGATCGAAGGCAAACATCGGGT
GCGTCGCAGAGCCGAGCTAGCTGGAGGACGTCGACTACGTTCTCTGTAGTTAAACGTTGT
ACAACGCCTTTTGAGCACCGTTGCTTCAGCTGGGTGACCATGTAGACGTGAGACAACGCT
NAAAGATGGATTCCATATCTCTCCATCTCATCCTCCGTCAAACTAAAACAGAGACGCANA
AGGGTAGTTTAGTCATTTCCTGACAGCAATGGCTTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 15-K113594-0022-464-F01C-8409
GenBank Accession LN493053 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K113594-0022-464-F01C-8409
ATTGAAGCCATTGCTGTCGGAAATGACTAAACTACCCTTTTGCGTCTCTGTTTTAGTTTG
ACGGAGGATGAGATGGAGAGATATGGAATCCATCTTTTAGCGTTGTCTCACGTCTACATG
GTCACCCAGCTGAAGCAACGGTGCTCAAAAGGCGTTGTACAACGTTTAACTACAGAGAAC
GTAGTCGACGTCCTCCAGCTAGCTCGGCTCTGCGACGCACCCGATGTTTGCCTTCGATCT
ATGCGTCTGATCCATTCGCAGTTTAAGACCGTTGAGCAGACTGAAGGATGGAAGTTTATT
CAAGAACATGATCCTTTCCTCGAGCTCGACATTCTCCAGTTCATCGATGACGCCGAATCG
GTAAACTTACTTATATGTTTTGAAAACACAGCATCTCTGTTTTTCTTAGTACCTTCGATA
ACACATTTTGTTCAGCTAGGTTTAGTTTTTGTTCC

Last Updated on 06.12.2018 16:11