Sequences from confirmation amplicon


Confirmation sequences for line 464G08 and hit At2g18180 are shown.
Line ID 464G08
Locus At2g18180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7911289
Primers used for PCR 10vy - 8474, show primer details
   
Sequence Name 03-K118356-0022-464-G08C-10vy
GenBank Accession LN487681 [GenBank]
Sequencing primer gene specific primer, 10vy: 5'-ATGGCTTTTGTAATGGGTGTAGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K118356-0022-464-G08C-10vy
ACGTTCAGGAAGCTAACCGAGTCAACTATATACTCTAGCCCGGTTTACTGCGACGAAAAC
TCGATGAACAAGAGCTCGATGCATGGGAAGAAAATGGCAACAACAACCATCTCAGGGGAA
GATTTCATGGCGGTCATGAAACGAATGGCTGAGCTTGAGCAGAAAGTCACAAACTTGAGT
GCACAGCCAGCAACTATGCCTCCTGAAAAGGAAGAGATGCTTAATGCTGCCATTAGCCGA
GCCGATTTCTTGGAACAAGAACTTGCCAAAAGAAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCACAAATTA
   
Sequence Name 03-K118355-0022-464-G08C-8409
GenBank Accession LN487680 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K118355-0022-464-G08C-8409
TTCTTGTTCCAAGAAATCGGCTCGGCTAATGGCAGCATTAAGCATCTCTTCCTTTTCAGG
AGGCATAGTTGCTGGCTGTGCACTCAAGTTTGTGACTTTCTGCTCAAGCTCAGCCATTCG
TTTCATGACCGCCATGAAATCTTCCCCTGAGATGGTTGTTGTTGCCATTTTCTTCCCATG
CATCGAGCTCTTGTTCATCGAGTTTTCGTCGCAGTAAACCGGGCTAGAGTATATAGTTGA
CTCGGTTAGCTTCCTTGGAACGTTTTTAGTCACTCTAATCATGGTGACTACACCCATACA
AAAAGCCAT

Last Updated on 06.12.2018 16:11