Sequences from confirmation amplicon


Confirmation sequences for line 465A03 and hit At5g20390 are shown.
Line ID 465A03
Locus At5g20390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6893060
Primers used for PCR 32xc - 8474, show primer details
   
Sequence Name 40-K119356-0022-465-A03C-32xc
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 32xc: 5'-CTCTCTCTGACTCGTTCAGTACCA-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K119356-0022-465-A03C-32xc
TGGTGTATACCCGAGAGGAAAGGTTGTTTATGTGCGTAGATAGGAGATGAGAAGAAGTGA
TTGTGGGGTCTCAAGGTAAATCTCTCCATTGTTGGTGTCACATAGACGACA
   
Sequence Name 20-K119355-0022-465-A03C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K119355-0022-465-A03C-8409
TCATTGAATATATACATGGCTTCAATTGATTCTCAAAAGCCAGCGGGAACCGAACAAAAT
TTTGGGTTATATAATCCAACAGATATGAAGCCCATCTACAAGATGTTTTGATTTTTAGAT
TCTTGATTTTATAGACTATCCCAAATCATTAGTAAATTAATGATGCTCTAATAGTTGTAA
TAGAGCGGGATAAAATCAAGATCTACAACAACATTCATTTTATATGTTGTATGATCTTCT
TAAATAAAAAAACTTAAGAAGCAGAACTCTAGCACCAGAAAAGATAACATCTTATTAGCT
ATGGACAAGTAAATTGGCACAATTTTTTCATTTTTTTTTTAAATTGGCACAATTTGGTAG
TCTACAACAAATATTACTAATTATTGTTTTTGACAAATTACTAACAGTGGCTGCTATATA
AATATATTTATACTATCCTAAATCATTATCTTAAAAATTAAAATGCTCTAATAATGTTTT
TTGCAATTTTTATATAGTAGTCATTAAAAAAAAAAAAAGGTGAAAACAAAAAAACCCCCC
AAGGGATCTTTTTAATCCAAAGGGAAAGGGGCCCCCCATGAAAAACAATCCGCCCCACGG
GGAAACCCAAAAAATTTTTTTTTTTCCCCCCCGGGGCCCCCCTTTCCCCCTTTTTTTTTT
TTAAATTTAGGGAAAACATTCTCATTTTCCATTTTGGCTTCTTCTCCCTTTTCCCC

Last Updated on 06.12.2018 16:11