Sequences from confirmation amplicon


Confirmation sequences for line 465A12 and hit At2g22240 are shown.
Line ID 465A12
Locus At2g22240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9452048
Primers used for PCR 51ye - 8474, show primer details
   
Sequence Name 17-K120044-0022-465-A12C-51ye
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 51ye: 5'-TGAAAACTCGAGTCTGCGTAGTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K120044-0022-465-A12C-51ye
GTTATGATCTCGCTAATTTGGTAGAGGTTTTGGATTCTGAATCCCGATAGAGGATCACAG
ATCCTCTATAAAAAAGATAACCGCCATATGGTTGAGTATTAATGTCTTGAAGATCATTTC
ACTGAAAAGTCAGAGTTTGTGTATTGCAGGCAATGGCGCAAGTCGGAGGTATAGTAATGC
TGCAGCCGGAAGTAGGAGGCTCTCAAGATAACTTCTTCTTTGCTGGTATTGATAAAGTGA
GATTTCGCAAACCAGTGATTGCAGGAGACACGCTAGTCATGAGGATGACTCTTCTCAAGT
TCCAGAAGCGGTTCGGGCTCGCTAAAATGGAAGGTAAAGCTTATGTGGGTGGTGCTCTGG
TGTGTGAAGGCGAGTTCATGATGGTTTCTGCTGGTAGTTCTTGATCTCACCCATTAATGG
TCTCACTTCCCACATGTGGACTCTTCTTATGCTTCTTCTGTTATTCGAAAATCTAGACAA
GACAGCTTTATGTTTTCGACATTTATATGAGCTACTTTTGCCCTTCGTGTCTTTATTGCT
GTCTCCAATTTATGAACATTCATAAGATACAAATACAGAGATTTTGATTTTGAAAATCCA
TATTCATAGATCATAAGTTTGGTTATATTACAAGGAGACACAAGCATAATTACTTAAATC
TTTTACACATTTGATCCAAAAGCTTAGCTAATCTTCTCACCAAATAAACCGACCAGATCC
ATATCACACAAGTTAACCCTAAGCTTATCCATATTATCCGTCACACACACATTCATATAA
TATACATACAAAATGATATATATAAATGAAACAATGGAGCAGTGTCCTTCATATCCTAAA
GTCATCTTAGGTACCTTTACTTCTTCTTCCTCATCCTTCACTTGTACTCCATGATCATGT
TGTTCTCTGGCGCAAGCCCAACACATGCCCTAAAAATGTTT
   
Sequence Name 02-K120043-0022-465-A12C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K120043-0022-465-A12C-8409
ACTCAGTTCCTCACAAAGGCACCTCTTGTACCGCCCGGAACACCGGTGGTTAACGCTCTG
TCGAAGCAGCGGGCAATGCTGGAAAACATTCTTAGGGCATGTGTTGGGCTTGCGCCAGAG
AACAACATGATCATGGAGTACAAGTGAAGGATGAGGAAGAAGAAGTAAAGGTACCTAAGA
TGACCTTAGGATATGAAGGACACTGCTCCATTGTTTCATTTATATATATCATTTTGTATG
TATATTATATGAATGTGTGTGTGACGGATAATATGGATAAGCTTAGGGTTAACTTGTGTG
ATATGGATCTGGTCGGTTTATTTGGTGAGAAGATTAGCTAAGCTTTTGGATCAAATGTGT
AAAAGATTTAAGTAATTATGCTTGTGTCTCCTTGTAATATAACCAAACTTATGATCTATG
AATATGGATTTTCAAAATCAAAATCTCTGTATTTGTATCTTATGAATGTTCATAAATTGG
AGACAGCAATAAAGACACGAAGGGCAAAAGTAGCTCATATAAATGTCGAAAACATAAAGC
TGTCTTGTCTAGATTTTCGAATAACAGAAGAAGCATAAGAAGAGTCCACATGTGGGAAGT
GAGACCATTAATGGGTGAGATCAAGAACTACCAGCAGAAACCATCATGAACTCGCCTTCA
CACACCAGAGCACCACCCACATAAGCTTTACCTTCCATTTTAGCGAGCCCGAACCGCTNC
TGGAACTTGAGAAGAGTCATCCTCATGACTAGCGTGTCTCCTGCAATCACTGGTTTGCGA
AATCTCACTTTATCAATACCAGCAAAGAAGAAGTTATCTTGAGAGCCTCCTACTTCCGGC
TGCAGCATTACTATACCTCCCACTTGC

Last Updated on 06.12.2018 16:11