Sequences from confirmation amplicon


Confirmation sequences for line 465E04 and hit At3g01900 are shown.
Line ID 465E04
Locus At3g01900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:312393
Primers used for PCR 40hw - 8474, show primer details
   
Sequence Name 44-K112776-0022-465-E04C-40hw
GenBank Accession LN505239 [GenBank]
Sequencing primer gene specific primer, 40hw: 5'-TCATCATCCCAAGATGATAACAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K112776-0022-465-E04C-40hw
ACTGAGCTATATTGGTTGAATTTTCTTCAAACTCAAAAATTTAGTAGAGACTTTATGACT
TGGATTGAAAAAAAAAAAAAAAAGTTGGATTTTTTCCCCCAAGGGAAAAAATATTTTGGG
GTAAAAAGGACCAAAAACCCTTTTTCCAAAAAAAAAAACCCCTGGAAATTGGCCTTTGTT
GGTATTTCCAGGGGAAAAAAAAGGGTCCCCCCCAAAAACCCCATTTGAAAAAAAATTTTT
TTTCCAAAAAAGGGACCCCCCCATTTGAAAATTCCATTTGAAAGGGGCTCCTGGCCGGGA
AATCCCCCGGGGACCCCCATGGATTTGGGGGGCCATTTGGGAAAAAAAAAAAAATTATTC
CCCATTTTTTTTCCATTCCAAAATTGTAATTCCCCCCCCCGTTTTTAAAATTTTGGGGG
   
Sequence Name 21-K112774-0022-465-E04C-8409
GenBank Accession LN495735 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K112774-0022-465-E04C-8409
CATTTCATTGATATTCAATATGGAGAGATCCTTCTCTTGAAAGATATATTATTTTACAAT
TGCGTTCATCGGAGAGATCCTTCTCTTTCCCCTTGAATATACAAACAAAGTCAATTATCA
TGGCTTTTTATTTTATGTAAAACGTCTCTATGATCATTCATACCCAAAATTATTTTCTCC
TTTGGGTAAACAACTCAACTTTTTTTTTTTTTTTTCAACCCCAAGCAAAAAGCCCCCAAA
AAATTTTTGGATTTTGAAAAAAATTCCCCCCAATTTACCCCCATTTTTAATTTTTTTTTT
AAAAAAAACCCCTTTTGCCTTTTTTAACCCCCGGGGGAAAAAAAAAAAGGGAAAAGGGGG
TTTTTTTAAAAAAAAAATATAATCCGGGGGCAATTTGGGGTGAATTAAAAAAAAAAACAG
G

Last Updated on 06.12.2018 16:11