Sequences from confirmation amplicon


Confirmation sequences for line 466B10 and hit At5g62940 are shown.
Line ID 466B10
Locus At5g62940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25257424
Primers used for PCR HM86 - 8474, show primer details
   
Sequence Name 96-K101272-0022-466-B10C-8409
GenBank Accession LN515381 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>96-K101272-0022-466-B10C-8409
GAGCCATTTAAATTGAATATATCCTAATGGTGATGATCATAATTAGATCGCATAAGTCTT
TGGCATGTCGATATATCCATGAGACCACCACCATTTCCATTGCCTCCCGTAGAAATCCCA
TTAAATCCACCATTGTTGTTGTTGTTGTTAAGACCTACACCGTGATTCAGACCCATGTGA
TGATTATGATGATGATGATGATCTCCGTTACTTCCATGATTAACCATGACCAGGTTGTCA
TTGTTCACTCCCATGAGATCAAACTTACTGTCCAAGAAATCAATAGGTCTAGGGCTCTGC
GAAAGCAAACCGCCATACTTGCTCTCCAAGAAACCAACGTTACCATGCTCCGGAGTTGAG
TAAGACCCCATCATCCCACCAAAATGTGAAAACCCTAGAGGAGAATGTTGGTAATTTTGA
TGAGAATGTGCTAAAGCCATAAGATCAGAGGTATTGGCGGTAACGATGTTGGATGGTTTT
TTGCCGGAAGAAGTGGAGGAGGAGGAATTA
   
Sequence Name 96-K101278-0022-466-B10C-HM86
GenBank Accession LN515382 [GenBank]
Sequencing primer gene specific primer, HM86: 5'-CTAATTCCTCCTCCTCCACTTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>96-K101278-0022-466-B10C-HM86
TCACTCGTTCCGCCATACCTCTGATCTTATGGCTTTAGCACATTCTCATCAAAATTACCA
ACATTCTCCTCTAGGGTTTTCACATTTTGGTGGGATGATGGGGTCTTACTCAACTCCGGA
GCATGGTAACGTTGGTTTCTTGGAGAGCAAGTATGGCGGTTTGCTTTCGCAGAGCCCTAG
ACCTATTGATTTCTTGGACAGTAAGTTTGATCTCATGGGAGTGAACAATGACAACCTGGT
CATGGTTAATCATGGAAGTAACGGAGATCATCATCATCATCATAATCATCACATGGGTCT
GAATCACGGTGTAGGTCTTAACAACAACAACAACAATGGTGGATTTAATGGGATTTCTAC
GGGAGGCAATGGAAATGGTGGTGGTCTCATGGATATATCGACATGCCAAAGACTTATGCT
ATCTAATTATGATCATCACCATTAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC

Last Updated on 06.12.2018 16:11