Sequences from confirmation amplicon


Confirmation sequences for line 469C03 and hit At4g13620 are shown.
Line ID 469C03
Locus At4g13620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7932490
Primers used for PCR HF59 - 8474, show primer details
   
Sequence Name 73-K101672-0022-469-C03C-8409
GenBank Accession LN512865 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K101672-0022-469-C03C-8409
GCCATTTACATTGAATATACATGATGGATGAATAGAAGATGTGAGCTCATGATCATGAGG
CGACACAAAGTAGCTCGGAGTCGAAGAAGCAGGAAGAAGAGGAGATTTCATACCTTCCAA
AAATATTGGAACACCGAGAAGTTGTGGCGGCGTCGAAGAACTCAAGAACCCGTAGATTCC
AGAAGACAAGAGCTTGTCGTGCATGAAGCTAGTTTCTATTTTAGGAAACTGATCTTCCAT
GTTCCTTTTTTTGTTTGTTTTTTGAGTGTTTGTCCAGAGAATGAAGCCTGTTCTTAATTT
TGACAAAATAGGTGGAGAATAAATGTTTATATATACGAGAATTAAGGAGTGTTGTGTGTG
TATTGGTGTGATCATGTGGAGATAATATTAGAAAATTTAAGGAACCTAACTTTATTGTCC
GAAAAAAACATTATCATCTGACTCAGATCTACGCTCTGCCAAGCTTAATTGGTTCATATG
   
Sequence Name 73-K101679-0022-469-C03C-HF59
GenBank Accession LN512866 [GenBank]
Sequencing primer gene specific primer, HF59: 5'-CATATGAACAAATTAAGCTTGGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K101679-0022-469-C03C-HF59
ATATGTTTTTTTCGGACAATAAAGTTAGGTTCCTTAAATTTTCTAATATTATCTCCACAT
GATCACACCAATACACACACAACACTCCTTAATTCTCGTATATATAAACATTTATTCTCC
ACCTATTTTGTCAAAATTAAGAACAGGCTTCATTCTCTGGACAAACACTCAAAAAACAAA
CAAAAAAAGGAACATGGAAGATCAGTTTCCTAAAATAGAAACTAGCTTCATGCACGACAA
GCTCTTGTCTTCTGGAATCTACGGGTTCTTGAGTTCTTCGACGCCGCCACAACTTCTCGG
TGTTCCAATATTTTTGGAAGGTATGAAATCTCCTCTTCTTCCTGCTTCTTCGACTCCAAG
CTACTTTGTGTCGCCTCATGATCATGAGCTCACATCTTCTATTCATCCATCAGGTATATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCA

Last Updated on 06.12.2018 16:11