Sequences from confirmation amplicon


Confirmation sequences for line 469F01 and hit At5g14860 are shown.
Line ID 469F01
Locus At5g14860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4807044
Primers used for PCR HF60 - 8474, show primer details
   
Sequence Name 30-K101424-0022-469-F01C-8409
GenBank Accession LN499649 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K101424-0022-469-F01C-8409
AAGAATTGACCTTTTTAAACAATCAAGACCACGTAAATTCACAAAACAATACTTTTCTTT
ATATATATATATATAGACTAAAAAATGTTTTATGGGTTGATACCAAAAACAAATAAAAGG
ACATGCATGGAATTTGGTGGAATAATAAAAGTGATTGGTCAACTGTATTAATATGAGGTT
TTAAGTTGGCATTAATCCATTCTTTAACGTACCTTGGAATCTTCCAACCCTAATGCTATT
TCCTTGAGCTGCTCGTTCGATATCTCAGCCTGCGTTCCAAACGCCACATACATAACCGGA
CATCTTTCCTCTAGTTTCCGGTCCAACCAATGAATCCAATCCGGTTTATCACTCTCCGGT
TTTGGAGGATTTACCAAACACAAAGGCCCAACACACCACGGTTTTGGTTCATCGTTATCA
CGGAGCCGGTAGTCAACGAACGTTGACTCGAGCTCGTAAAAGCTGTTCACTATAACTCCA
CGGCTTTTCTTGGTGGACATAAGATGGTCAATGAGTAGCTCGAACGCTGGATCCGATTGA
TCCGGTTCGGTCAAAACCGGATCGAACTCACACTTCTTAACACATATCCGTGGAAAATCC
GGTACAGTAACCGGTTCAGTATCAGATTTAACACTTTCCGGTTTGGT
   
Sequence Name 70-K101427-0022-469-F01C-HF60
GenBank Accession LN512351 [GenBank]
Sequencing primer gene specific primer, HF60: 5'-TACCAAACCGGAAAGTGTTAAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K101427-0022-469-F01C-HF60
GTGTTAAGAAGTGTGAGTTCGATCCGGTTTTGACCGAACCGGATCAATCGGATCCAGCGT
TCGAGCTACTCATTGACCATCTTATGTCCACCAAGAAAAGCCGTGGAGTTATAGTGAACA
GCTTTTACGAGCTCGAGTCAACGTTCGTTGACTACCGGCTCCGTGATAACGATGAACCAA
AACCGTGGTGTGTTGGGCCTTTGTGTTTGGTAAATCCTCCAAAACCGGAGAGTGATAAAC
CGGATTGGATTCATTGGTTGGACCGGAAACTAGAGGAAAGATGTCCGGTTATGTATGTGG
CGTTTGGAACGCAGGCTGAGATATCGAACGAGCAGCTCAAGGAAATAGCATTAGGGTTGG
AAGATTCCAAGGTACGTTAAAGAATGGATTAATGCCAACTTAAAACCTCATATTAATACA
GTTGACCAATCACTTTTATTATTCCACCAAATTCCATGCATGTCCTTTTATTTGTTTTTG
GTATCAACCCATAAAACATTTTTTAGTCTATATATATATATATAAAGAAAAGTATTGTTT
TGTGAATTTACGTGGTCTTGATTGTTTTAAAAAGGTCAATTTCTTGTGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAAGAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTTCAAAATGGAGATGTCCGC

Last Updated on 06.12.2018 16:11