Sequences from confirmation amplicon


Confirmation sequences for line 469F05 and hit At2g35380 are shown.
Line ID 469F05
Locus At2g35380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14893715
Primers used for PCR 43xe - 8474, show primer details
   
Sequence Name 46-K119373-0022-469-F05C-43xe
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 43xe: 5'-ATCGAAACCTATGTGGTCAGTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K119373-0022-469-F05C-43xe
TGGCCCATGGTGGGAAGTTTTGCTAGGGAGAAGAGACTCATTAAAGGCAAGTTTCGCCGG
AGCAAACCAATTCATTCCAGCACCAAACTCCTCTCTCGACAGCCTCATCATCAATTTCAA
GCAACAAGGGCTCAACATTCAAGATCTCATCGCTCTTTCGGGTGCTCATACAATAGGTAA
AGCGAGATGCGTGAGCTTCAAGCAACGCATTGTTCAACCAAACATGGAACAAACGTTCTA
CGTCGATGAGTTCAGAAGACACAGCACGTTCCGTCGAGTCCTAGGGTCACAATGCAAAGA
TTCAAGCCGAGACAACGAACTGTCTCCACTGGATATTAAGACACCAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATATC
   
Sequence Name 14-K119372-0022-469-F05C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K119372-0022-469-F05C-8409
CCTTGGTGTCTAATATCCAGTGGAGACAGTTCGTTGTCTCGGCTTGAATCTTTGCATTGT
GACCCTAGGACTCGACGGAACGTGCTGTGTCTTCTGAACTCATCGACGTAGAACGTTTGT
TCCATGTTTGGTTGAACAATGCGTTGCTTGAAGCTCACGCATCTCGCTTTACCTATTGTA
TGAGCACCCGAAAGAGCGATGAGATCTTGAATGTTGAGCCCTTGTTGCTTGAAATTGATG
ATGAGGCTGTCGAGAGAGGAGTTTGGTGCTGGAATGAATTGGTTTGCTCCGGCGAAACTT
GCCTTTAATGAGTCTCTTCTCCCTAGCAAAACTTCCCACCATGGGCCACCTCTCTGATGA
TACCAAACAATATTTGTGTTTAAATGAAACTGACCACATAGGATTTTCG

Last Updated on 06.12.2018 16:11