Sequences from confirmation amplicon


Confirmation sequences for line 469F06 and hit At4g19030 are shown.
Line ID 469F06
Locus At4g19030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10423230
Primers used for PCR KF35 - 8474, show primer details
   
Sequence Name 51-K103224-0022-469-F06C-KF35
GenBank Accession LN507468 [GenBank]
Sequencing primer gene specific primer, KF35: 5'-ATGCACGAATGATATAGGCTCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K103224-0022-469-F06C-KF35
ATTCTGCATGTAGCATTCTAAAATTTTCTATAAATTTTANATTTGTTGATCTAAGAAAAA
ATTGTAATCCCTGAAAATTGAATTTGAGACCTGGTATAGAATCTCCACAAACATAGGAAT
TTATTATTATTAAAAAAGCTTTATTAACAATATATCATCTTCTTCTTTTTTTCAATTAAT
AAACTGGTAATATTAAAATCGTCAAAGAAGTCTCTCTCTCCCCCATGTTTGCCAATAAAT
AGGAGATAATCGCTACCAAACTTCTCATTCTCACAAGTCATAAACAGTCGTTTATAGTTC
AATCCTTTTCCTCTTCTTTGTTTCTAAAAAGAGAAAAGTCACTTATGGCGGATATCTCGG
GAAACGGCTATGGCAACGCCNGAGAAGAAGTAGTAATGGTGAATCTCANGGATGAAGTCG
AACATCAACAAGAAATGGAAGACATTCATAACCCAAGACCCTTGAAGAAACAAGACTCTC
TACTCTCAGTCTCTGTCCCTTTCTTACAAAAGGTTTTTACTTTTCAGGATATATTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATTTCCG
   
Sequence Name 19-K103225-0022-469-F06C-8409
GenBank Accession LN494719 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K103225-0022-469-F06C-8409
TCGGAATGAGCCATTTACATTGAATATATCCTGAAAAGTAAAAACCTTTTGTAAGAAAGG
GACAGAGACTGAGAGTAGAGAGTCTTGTTTCTTCAAGGGTCTTGGGTTATGAATGTCTTC
CATTTCTTGTTGATGTTCGACTTCATCCTTGAGATTCACCATTACTACTTCTTCTCTGGC
GTTGCCATAGCCGTTTCCCGAGATATCCGCCATAAGTGACTTTTCTCTTTTTAGAAACAA
AGAAGAGGAAAAGGATTGAACTATAAACGACTGTTTATGACTTGTGAGAATGAGAAGTTT
GGTAGCGATTATCTCCTATTTATTGGCAAACATGGGGGAGAGAGAGACTTCTTTGACGAT
TTTAATATTACCAGTTTATTAATTGAAAAAAAGAAGAAGATGATATATTGTTAATAAAGC
TTTTTTAATAATAATAAATTCCTATGTTTGTGGAGATTCTATACCAGGTCTCAAATTCAA
TTTTCAGGGATTACAATTTTTTCTTAGATCAACAAATATAAAATTTATCGAAAATTTTAG
AATGCTACATGCAGAATTTGTTTGTTTTGATCTTGTGTGTTGTGCAGAGAGCCTAT

Last Updated on 06.12.2018 16:11