Sequences from confirmation amplicon


Confirmation sequences for line 469F11 and hit At1g70500 are shown.
Line ID 469F11
Locus At1g70500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26567803
Primers used for PCR 13di - 8474, show primer details
   
Sequence Name 66-K117069-0022-469-F11C-13di
GenBank Accession LN511694 [GenBank]
Sequencing primer gene specific primer, 13di: 5'-TCTGCACCAAACTCTGTTTTATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K117069-0022-469-F11C-13di
TGGTCCATGTCAAGAAAAATTGATCATTCAGGTAATCTTTTAACTTCTTAATTCCTGTTA
CACGTTAAGCTACAACAACATGTTACCATTTATTGTCTTATGCATTTTTATTTTTTTTTA
ATTTTGATTTTGATAGATTGATGGAACAATCATAGCACCAGATGAGCCTAGCCAATGGGA
CCCGAAATTCCCAAGGAATTGGCTTCAATTTTCCAAACTCCAAGGAGTTGTATTCCAAGG
GAATGGAGTTATAGATGGTTCTGGTACCAAATGGTGGGCTGCTTCTTGCAAGAAGAACAA
GTCTAACGTAAGTATCTTCTTTACATAAAGTGGACTTTCTTCTAATCAATCATGAAGTAG
AAATCTGACACATATCTTTCTTGTTTTTCTTTTGTAGCCTTGCGTAGGCGCACCAACGGT
CTAAGTTTTTTCCCTTTTGTGTTCATCGACCATTAGACTTATCAGCAATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGT
   
Sequence Name 45-K117068-0022-469-F11C-8409
GenBank Accession LN505663 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K117068-0022-469-F11C-8409
GTCTAATGGTCGATGAACACAAAAGGGAAAAAACTTAGACCGTTGGTGCGCCTACGCAAG
GCTACAAAAGAAAAACAAGAAAGATATGTGTCAGATTTCTACTTCATGATTGATTAGAAG
AAAGTCCACTTTATGTAAAGAAGATACTTACGTTAGACTTGTTCTTCTTGCAAGAAGCAG
CCCACCATTTGGTACCAGAACCATCTATAACTCCATTCCCTTGGAATACAACTCCTTGGA
GTTTGGAAAATTGAAGCCAATTCCTTGGGAATTTCGGGTCCCATTGGCTAGGCTCATCTG
GTGCTATGATTGTTCCATCAATCTATCAAAATCAAAATTAAAAAAAAATAAAAATGCATA
AGACAATAAATGGTAACATGTTGTTGTAGCTTAACGTGTAACAGGAATTAAGAAGTTAAA
AGATTACCTGAATGATCAATTTTTCTTGACATGGACCATCGAATTTCGTCGCATTTACTA
AATAGCTACGTCCTTGTGGAACCAATAAAA

Last Updated on 06.12.2018 16:11