Sequences from confirmation amplicon


Confirmation sequences for line 470B09 and hit At2g14520 are shown.
Line ID 470B09
Locus At2g14520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:6183226
Primers used for PCR 31kh - 8474, show primer details
   
Sequence Name 82-K113669-0022-470-B09C-31kh
GenBank Accession LN514218 [GenBank]
Sequencing primer gene specific primer, 31kh: 5'-GGTAAAGGTGGAGAATTGACACAT-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K113669-0022-470-B09C-31kh
CTGAGAAATGGCTAAGATGCAATGACACCAATTTCTGACACTTTTGTGATTGATATCAAT
GCCAAACTAGACAGGTCTTGGTTTGTTCAACCCTCTTCGCACATTCCTTAATCCTCTAAA
TCATACAAAACTTAGTTTCTTTTTCCTTCGCCAGGGATTTGATGAATTTGATTCTTGATA
AAGGTCATAGCAGAGTCCCAGTATACTATGAGCAACGTACCAACATAATTGGACTTGTTT
TGGCTACATGTCCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAG
   
Sequence Name 51-K113666-0022-470-B09C-8409
GenBank Accession LN507566 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K113666-0022-470-B09C-8409
TTGAATATATCCTGGGGACATGTAGCCAAAACAAGTCCAATTATGTTGGTACGTTGCTCA
TAGTATACTGGGACTCTGCTATGACCTTTATCAAGAATCAAATTCATCAAATCCCTGGCG
AAGGAAAAAGAAACTAAGTTTTGTATGATTTAGAGGATTAAGGAATGTGCGAAGAGGGTT
GAACAAACCAAGACCTGTCTAGTTTGGCATTGATATCAATCACAAAAGTGTCAGAAATTG
GTGTCATTGCATCTTTAGCCATTTTCTCAGATAGTTCAAGAGCTCCAGCAATGATTGTTG
TTTCATCATGTGTCAATTCTCCACCTTTACCA

Last Updated on 06.12.2018 16:11