Sequences from confirmation amplicon


Confirmation sequences for line 470E07 and hit At4g11260 are shown.
Line ID 470E07
Locus At4g11260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6853924
Primers used for PCR BD47 - 8474, show primer details
   
Sequence Name 46-K028099-022-470-E07C-8409
GenBank Accession LN505751 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K028099-022-470-E07C-8409
ATTTTCTTTTGATTTTATTTTTTAACATAAGATTGATTCTTGATAAGTCCAGAATTTTTT
AACAAATAAATTAGTTGACAAAAGAATAATACTGAAAAAAGGAAAGAAAACTAATGGTAA
AGATATTTATAGAGAACTACAATAATTTAAAATAATCATTTTCTACTCATTTTGTTTATA
TCGTAATCGTTTACTCATAGTTTAGCAAATACAATGTGATTTGTGAATTTAGACTATGAT
ATATTTTAACAAGTTATAATTTATATATTGACTAGATTAGGACCCGTCGTCCAATACACA
GATTTAAGTATTTTAAGTTGATAATAATCTAGTAAAGTTAAAATATCAATTATCAAACTT
TTGCCAAATATTTACCTTAAAAAATTTATTTGCAACCTCGAAAAATCCATCTAAATATGT
ACAGATATATTTGTCGTGATTACTGTTACGACGTCGTTTACCAGTTC
   
Sequence Name 46-K028099-022-470-E07C-BD47
GenBank Accession LN505752 [GenBank]
Sequencing primer gene specific primer, BD47: 5'-GAAGAAGGAAGAACGAACTGGTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K028099-022-470-E07C-BD47
TATCTGTACATATTTAGATGGATTTTTCGAGGTTGCAAATAAATTTTTTAAGGTAAATAT
TTGGCAAAAGTTTGATAATTGATATTTTAACTTTACTAGATTATTATCAACTTAAAATAC
TTAAATCTGTGTATTGGACGACGGGTCCTAATCTAGTCAATATATAAATTATAACTTGTT
AAAATATATCATAGTCTAAATTCACAAATCACATTGTATTTGCTAAACTATGAGTAAACG
ATTACGATATAAACAAAATGAGTAGAAAATGATTATTTTAAATTATTGTAGTTCTCTATA
AATATCTTTACCATTAGTTTTCTTTCCTTTTTTCAGTATTATTCTTTTGTCAACTAATTT
ATTTGTTAAAAAATCTGGACTTATCAAGAATCAATCTTATGTTAAAAAATAAAATCAAAA
GGAAATTAGAAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGT
CGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACG
TGTCTACATT

Last Updated on 06.12.2018 16:11