Sequences from confirmation amplicon


Confirmation sequences for line 471D08 and hit At3g55410 are shown.
Line ID 471D08
Locus At3g55410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20544820
Primers used for PCR 10ie - 8474, show primer details
   
Sequence Name 35-K112875-0022-471-D08C-8409
GenBank Accession LN501895 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K112875-0022-471-D08C-8409
ATTTCATTGAATAATCCTGCGAAAGCAATTCAAGAATGTAATTGGCAGATTCTCTATGCC
ACAACTCCCGCCAACTATTTCCATGTTCTGCGGCGACAGGTAAGACCTATTTCTGATTAT
CTCTTTTACCGGTCACCAGGTGTTGTTTCCAGCTTACAATTTCTTTATGTCACTCATGTT
GCAGATACACAGAGACTTCCGTAAGCCTCTGATTGTAATGGCACCAAAGAACTTGCTCCG
TCACAAGGACTGCAAATCAAATCTCTCAGAGTTTGATGATGTCCAAGGCCACCCAGGTTT
TGACAAGCAAGGAACTAGATTTAAGCGATTAATCAAGGATCAGAATGATCACTCTGATCT
TGAAGAAGGCATCAGAAGATTGGTACTTTGCTCCGGNAAGGTATAAA
   
Sequence Name 51-K112876-0022-471-D08C-10ie
GenBank Accession LN507556 [GenBank]
Sequencing primer gene specific primer, 10ie: 5'-ATTTATACCTTTCCGGAGCAAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K112876-0022-471-D08C-10ie
TTCTGATCCTTGATAATCGCTTAATCTAGTTCCTTGCTTGTCAAAACCTGGGTGGCCTTG
GACATCATCAAACTCTGAGAGATTTGATTTGCAGTCCTTGTGACGGAGCAAGTTCTTTGG
TGCCATTACAATCAGAGGCTTACGGAAGTCTCTGTGTATCTGCAACATGAGTGACATAAA
GAAATTGTAAGCTGGAAACAACACCTGGTGACCGGTAAAAGAGATAATCAGAAATAGGTC
TTACCTGTCGCCGCAGAACATGGAAATAGTTGGCGGGAGTTGTGGCATTGACAATCTGCC
AATTACATTCTTGAATTTGCTTTCGCAGGATATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11