Sequences from confirmation amplicon


Confirmation sequences for line 471E12 and hit At4g25740 are shown.
Line ID 471E12
Locus At4g25740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13107927
Primers used for PCR 23wx - 8474, show primer details
   
Sequence Name 37-K118924-0022-471-E12C-23wx
GenBank Accession LN502796 [GenBank]
Sequencing primer gene specific primer, 23wx: 5'-ACACGCTGTTAGACAATCTCTTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K118924-0022-471-E12C-23wx
CTAGGAGTGATTAGTAAGTGGAAGGCATATAGGCTCAGTAAACTAGGAGTGATTAGTAAG
TCGAAGATTTACTTGGAAAGACGGCTGGAAATCAGCTGGAGCTCCACCCTTCTCATCACC
ACCACGTGGGCCTCCACGGTATCCATCACGGTCACCAAATCTGGGACGGTCTCCATCAGA
GCGAGGTGGTCCTCTGAAAACAAAAGCCAAAACAATATCAGAAATTAGCCAATCCCTCAG
ACCGGACAGGTCAAGGGATTTAAAGATGAAACTACCTGGATATATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGATTATTAT
AT
   
Sequence Name 13-K118923-0022-471-E12C-8409
GenBank Accession LN492273 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K118923-0022-471-E12C-8409
CATTTCATTGAATATATCCAGGTAGTTTCATCTTTAAATCCCTTGACCTGTCCGGTCTGA
GGGATTGGCTAATTTCTGATATTGTTTTGGCTTTTGTTTTCAGAGGACCACCTCGCTCTG
ATGGAGACCGTCCCAGATTTGGTGACCGTGATGGATACCGTGGAGGCCCACGTGGTGGTG
ATGAGAAGGGTGGAGCTCCAGCTGATTTCCAGCCGTCTTTCCAAGTAAATCTTCGACTTA
CTAATCACTCCTAGTTTACTGAGCCTATATGCCTTCCACTTACTAATCACTCCTAGTTAA
CTGTGCCAATATGCTTCGTTTAAAGTCTTTTAGCTAGTTGATGAAGAGATTGTCTAACAG
CGTG

Last Updated on 06.12.2018 16:11