Sequences from confirmation amplicon


Confirmation sequences for line 472F03 and hit At3g09520 are shown.
Line ID 472F03
Locus At3g09520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2924345
Primers used for PCR 31aq - 8474, show primer details
   
Sequence Name 16-K106755-0022-472-F03C-8409
GenBank Accession LN493447 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K106755-0022-472-F03C-8409
CGATTTCTATGCTTTATAGATGCTTAGACACTCCTTAGCGTAACCAGAACCGATCATGCA
CTCAGCGATGGATTTCAAATCCGTCATGACGTTGGTAGAAACCTCCTCCAGTTCGATGAT
CGTATCTAGATCTTCGACATAGTCGGGGAAATCTGAGTAGCTAGATCTTGCGGAAGTAAG
TGAAGATCGAGTGGACACTGATTCTGGATCAAGATAAGCACGATTCATCGAAAGAATCTG
GTAAAACTCTTTTTGCAACCTCTTCATAGCGATTTGCATAAGCTTATGCGATCTCAAGAG
TCTCTCAGAGTTTGGATCCTCA
   
Sequence Name 32-K106756-0022-472-F03C-31aq
GenBank Accession LN500601 [GenBank]
Sequencing primer gene specific primer, 31aq: 5'-GTGAGGATCCAAACTCTGAGAGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K106756-0022-472-F03C-31aq
CAAAAAGAGTTTTACCAGATTCTTTCGATGAATCGTGCTTATCTTGATCCAGAATCAGTG
TCCACTCGATCTTCACTTACTTCCGCAAGATCTAGCTACTCAGATTTCCCCGACTATGTC
GAAGATCTAGATACGATCATCGAACTGGAGGAGGTTTCTACCAACGTCATGACGGATTTG
AAATCCATCGCTGAGTGCATGATCGGTTCTGGTTACGCTAAGGAGTGTCTAAGCATCTAT
AAAAGCATTAGGAAATCGATCATCGACCGAAGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
Primers used for PCR 08bd - 8474, show primer details
   
Sequence Name 57-K118893-0022-472-F03C-08bd
GenBank Accession LN509559 [GenBank]
Sequencing primer gene specific primer, 08bd: 5'-AAACGAGAAGATAGACTCAATCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K118893-0022-472-F03C-08bd
CAGCAGCCGGAAACTTTCTCCGGCGGAGAGTGTTTCTTGCTAGTTTTAGTATTAATGATC
TCCGGGAATCCAAAGAGAAGAAGCGCACCGTCTCGAGAGATATCACTAAAGCAAGACTCT
CTAATTGCGTCAGAGGATTCAAAGACATGATCACACAAAATCTTCTCACCTTTGAAAAGA
GTCTCCATAGAAACTTTGACTGCTTTTAACCAGCTTCTGATCTTCAACTCCATAACTTCC
CATGACATTTTCTTCACCTTCCCAGTACTCGTCTTCTCCACTTCTAAACGATAAATCCCT
TTTCAACTGCATGCATGTCGACTCTAGAGGATCCCCAATTCCCATGGAGTCAAAGATTCA
AATAGAGGACCTAACAGAACTCGCCGTAAAGACTGGCGAACAGTTCATACAGAGTCTCTT
ACGACTCAATGACAAGAAGAAAATCTTCGTCAACATGGTGGAGCACGACACGCTTGTCTA
CTCCAAAAATATCAAAGATACAGTCTCAGAAGACCAAAGGGCAATTGAGACTTTTCAACA
AAGGGTAATATCCGGAAACCTCCTCGGATTCCATTGCCCAGCTATCTGTCACTTTATTGT
GAAGATAGTGGAAAAGGAAGGTGGCTCCTACAAATGCCATCATTGCGATAAAGGAAAGGC
CATCGTTGAAGATGCCTCTGCCGACAGTGGTCCCAAAGATGGACCCCCACCCACGAGGAG
CATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGATAT
CTCCACTGACGTAAGGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTAT
ATAAGGAAGTTCATTT
   
Sequence Name 17-K118892-0022-472-F03C-8409
GenBank Accession LN494079 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K118892-0022-472-F03C-8409
ATTGAATATATCCTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTA
CCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATA
GTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGA
AGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGGGGTCCATCTTT
GGGACCACTGTCGGCAGAGGCATCTTCAACGATGGCCTTTCCTTTATCGCAATGATGGCA
TTTGTAGGAGCCACCTTCCTTTTCCACTATCTTCACAATAAAGTGACAGATAGCTGGGCA
ATGGAATCCGAGGAGGTTTCCGGATATTACCCTTTGTTGAAAAGTCTCAATTGCCCTTTG
GTCTTCTGAGACTGTATCTTTGATATTTTTGGAGTAGACAAGCGTGTCGTGCTCCACCAT
GTTGACGAAGATTTTCTTCTTGTCATTGAGTCGTAAGAGACTCTGTATGAACTGTTCGCC
AGTCTTTACGGCGAGTTCTGTTAGGTCCTCTATTTGAATCTTTGACTCCATGGGAATTGG
GGATCCTCTAGAGTCGACATGCATGCAGTTGAAAAGGGATTTATCGTTTAGAAGTGGAGA
AGACGAGTACTGGGAAGGTGAAGAAAATGTCATGGGAAGTTATGGAGTTGAAGATCAGAA
GCTGGTTAAAAGCAGTCAAAGTTTCTATGGAGACTCTTTTTCAAGGTGAGAAGATTTTGT
GTGATCATGTCTTTGAATCCTCTGACGCAATTAAAGAGTCTTGCTTTAGTGATATCTCTC
GAGACGGTGCGCTTCTTCTCTTTGGATTCCCGGAGATCATTAATACTAAAACTAGCA

Last Updated on 06.12.2018 16:11