Sequences from confirmation amplicon


Confirmation sequences for line 472G06 and hit At3g54050 are shown.
Line ID 472G06
Locus At3g54050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20017359
Primers used for PCR PB23 - 8474, show primer details
   
Sequence Name 40-K119832w-0022-472-G06C-PB23
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, PB23: 5'-AGATAAGTGGAACCCACATGAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K119832w-0022-472-G06C-PB23
CGATCCTCTCTCCCTCCTCCAAACCAAACCTTCCAAAGTTTTTTTCAAAACAACACAGAA
AAAATGGCAGCAACCGCCGCAACAACGACGTCGTCTCACCTTCTTCTCTCAAGCTCACGT
CACGTGGCATCATCATCCCAGCCTTCAATCCTTTCACCGAGATCTCTCTTCTCCAACAAC
GGGAAACGAGCACCAACTGGAGTGAGGAACCATCAGTATGCGAGTGGAGTGAGGTGTATG
GCCGTAGCGGCGGATGCTGCTGAGACGAAAACGGCGGCGAGGAAGAAGAGTGGATACGAA
CTTCAAACGTTGACGGGCTGGTTGCTGAGACAAGAGATGAAAGGAGAGATAGATGCAGAG
CTGACGATAGTGATGTCGAGTATATCATTGGCTTGTAAGCAGATCGCTTCACTTGTTCAA
CGTGCCGGAATCTCTAACCTGACCGGAGTTCAAGGCGCCGTTAATATTCAGGATATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCACGGTTATTAT
   
Sequence Name 16-K119831w-0022-472-G06C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K119831w-0022-472-G06C-8409
TCGGATGAGCATTTCATTGAATATATCCTGAATATAACGGCGCCTTGAACTCCGGTCAGG
TTAGAGATTCCGGCACGTTGAACAAGTGAAGCGATCTGCTTACAAGCCAATGATATACTC
GACATCACTATCGTCAGCTCTGCATCTATCTCTCCTTTCATCTCTTGTCTCAGCAACCAG
CCCGTCAACGTTTGAAGTTCGTATCCACTCTTCTTCCTCGCCGCCGTTTTCGTCTCAGCA
GCATCCGCCGCTACGGCCATACACCTCACTCCACTCGCATACTGATGGTTCCTCACTCCA
GTTGGTGCTCGTTTCCCGTTGTTGGAGAAGAGAGATCTCGGTGAAAGGATTGAAGGCTGG
GATGATGATGCCACGTGACGTGAGCTTGAGAGAAGAAGGTGAGACGACGTCGTTGTTGCG
GCGGTTGCTGCCATTTTTTCTGTGTTGTTTTGAAAAAAACTTTGGAAGGTTTGGTTTTGG
AGGAGGGAGAGAGGATCGGATATTCGGATTGTGAGGTGGACGAAGGATTCGATTCATGTG
GGTTCCACTTATC

Last Updated on 06.12.2018 16:11