Sequences from confirmation amplicon


Confirmation sequences for line 472H07 and hit At5g04540 are shown.
Line ID 472H07
Locus At5g04540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1298145
Primers used for PCR 26qz - 8474, show primer details
   
Sequence Name 71-K115869-0022-472-H07C-26qz
GenBank Accession LN512613 [GenBank]
Sequencing primer gene specific primer, 26qz: 5'-TGCATAAGCAGCCCTTATAAGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K115869-0022-472-H07C-26qz
TTCTGACTTCACATCAGGCTCTTGTCGAAAAGGATTGGTTATCATTTGGTCACCCATTTT
CGGATCGGGTGGGAATGCCAAACGTGTCTGAATCTGGTAATTTTGAATTACCAATTCAGT
CTTCTTCTGCTCGAAGTTTTCCTTCATCTCCAGTGCGGCAATCTCCAGGATCAGCAGCTG
CTCAATCCTCTAGCTCTTCATATGGGCTGAACAACTATTCTCCTATATTTTTGCAGGTGA
CCAGCTATTACCTGCAATTATGTTATTCCTTTTGATTTGGAAAATCTACATGGATCAGCA
ATGATATCATGTTCACGTTAGCTGAGTATGATATAATCTACATGGAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 30-K115867-0022-472-H07C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K115867-0022-472-H07C-8409
TGTACTATCTTAGTAAAAGGCTCCCCATGCTAC

Last Updated on 06.12.2018 16:11