Sequences from confirmation amplicon


Confirmation sequences for line 473H09 and hit At5g51105 are shown.
Line ID 473H09
Locus At5g51105 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20777705
Primers used for PCR 19aj - 8474, show primer details
   
Sequence Name 16-K106540-0022-473-H09C-8409
GenBank Accession LN493442 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K106540-0022-473-H09C-8409
TCGGACATGAGCCTGTAGTTTCCCCCAATCATGTTCCCGTTTCCAATCCGTTCAAACCAA
GTCCCGGTATGGCAGGAAGTCCCGACTTGAGCAAATGTTGGTCAACAGTGATGGATTTGC
CGGGATGCTTTGAAGAGATTCAACAAGCCGTAATGTCCGGCAAATTTGAAGGCGTTGGTC
CTGCTTGTTGTAAAGCTTTCCTTGACGCTGAAGCTAACTGCTTACCAAATCTTCCATCCA
ACCCGTTTTTCCCTCCTATGATGAAACACCAATGCTCCAAAATGGCTAGTCCTCCAACCA
CCACCGCCTATTAGAATGCAAAAATGTAGTTGTGTTTTTTCGAATTTTTTGTTAGTCCCA
GAATGTTCTAGAAGAAGAAATGATAATAATAATGTCTTAATCTAATAATAATGTCTCATT
TCTTATATTGTGCTCCATATGATTGATGTTCTTTCTTCTTTTGATTGATGAATGAATCTC
AAAGACATGTTG
   
Sequence Name 32-K106541-0022-473-H09C-19aj
GenBank Accession LN500596 [GenBank]
Sequencing primer gene specific primer, 19aj: 5'-GCAACATGTCTTTGAGATTCATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K106541-0022-473-H09C-19aj
TCATCATATGGAGCACAATATAAGAAATGAGACATTATTATTAGATTAAGACATTATTAT
TATCATTTCTTCTTCTAGAACATTCTGGGACTAACAAAAAATTCGAAAAAACACAACTAC
ATTTTTGCATTCTAATAGGCGGTGGTGGTTGGAGGACTAGCCATTTTGGAGCATTGGTGT
TTCATCATAGGAGGGAAAAACGGGTTGGATGGAAGATTTGGTAAGCAGTTAGCTTCAGCG
TCAAGGAAAGCTTTACAACAAGCAGGACCAACGCCTTCAAATTTGCCGGACATTACGGCT
TGTTGAATCTCTTCAAAGCATCCCGGCAAATCCATCACTGTTGACCAACATTTGCTCAAG
TCGGGACTTCCTGCCATACCGGGACTTGGTTTGAACGGATTTGGAAACGGGAACATGATT
GGGGGAAACTACATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCACGGTTATTA

Last Updated on 06.12.2018 16:11