Sequences from confirmation amplicon


Confirmation sequences for line 474A08 and hit At5g58850 are shown.
Line ID 474A08
Locus At5g58850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23764326
Primers used for PCR 07md - 8474, show primer details
   
Sequence Name 53-K114180-0022-474-A08C-07md
GenBank Accession LN508225 [GenBank]
Sequencing primer gene specific primer, 07md: 5'-GAATTTAATGGGTCATTCATTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K114180-0022-474-A08C-07md
ATTCCTTATGACTAGCTAGCTAACTATCTCCTTTGATTTGTGCTCTCCTCTCCTTTTGAC
TATTCTATTCTTCTTCATTTTTTCCATGGAAGACCGACGCCTTGTCCATGGAGCTGCGCC
GCCGTTGACAGCCGTGGAGAGATTCTTATACGGTCAAAAGAATGATGCCTTGTGTTCCAA
GAAACAAGAGAGTTCTAGAGACCAACCGATCGTGAAGACAAAAATATCGATTGAGACTAG
AAGCGACAACAAAGAGAACACAACGTTTGGGCCGACGAGGGAGAAACACTTGGTCCTTAA
TGGCGGCAACAGAAACCCTACTGGAGAAGTGGTCGCTAGAAGTGCAGCCAGAGATTATCA
AAACAGTACGAAGAAACGATCATCCAAGAACCTCATCAAAGGGCAATGGACGGCGGAAGA
AGACAGGTATATATAGATAAAATGTTTTATTGCATCTATACAAGAGTATGATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 17-K114177-0022-474-A08C-8409
GenBank Accession LN493941 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K114177-0022-474-A08C-8409
ACTCTTGTATAGATGCAATAAAACATTTTATCTATATATACCTGTCTTCTTCCGCCGTCC
ATTGCCCTTTGATGAGGTTCTTGGATGATCGTTTCTTCGTACTGTTTTGATAATCTCTGG
CTGCACTTCTAGCGACCACTTCTCCAGTAGGGTTTCTGTTGCCGCCATTAAGGACCAAGT
GTTTCTCCCTCGTCGGCCCAAACGTTGTGTTCTCTTTGTTGTCGCTTCTAGTCTCAATCG
ATATTTTTGTCTTCACGATCGGTTGGTCTCTAGAACTCTCTTGTTTCTTGGAACACAAGG
CATCATTCTTTTGACCGTATAAGAATCTCTCCACGGCTGTCAACGGCGGCGCAGCTCCAT
GGACAAGGCGTCGGTCTTCCATGGAAAAAATGAAGAAGAATAGAATAGTCAAAAGGAGAG
GAGAGCACAAATCAAAGGAGATAGTTTAGCTAGCTAGTCATAAGGAATATGAGAGAACTT
AATGAGTTGCTATATAAATTCAAACCAATGAATGACCCANTTAAATT

Last Updated on 06.12.2018 16:11