Sequences from confirmation amplicon


Confirmation sequences for line 474C02 and hit At2g33590 are shown.
Line ID 474C02
Locus At2g33590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14226291
Primers used for PCR 44by - 8474, show primer details
   
Sequence Name 79-K108065-0022-474-C02C-44by
GenBank Accession LN513835 [GenBank]
Sequencing primer gene specific primer, 44by: 5'-TTTGTTTGACTTTGACCATCTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K108065-0022-474-C02C-44by
TTTTATCATCGGAAAAGCATTAATATTTATGTTCAGCTTAAATCCACATTTGTTATGTTA
TTTTCTACAATGATTAACAAATTTATACAGAAACAAGACGTACCAATTGAGATAAAAGTT
TGTGTGTTTTATCCTTTGAAAGTAAGATTTCGGTAGAAATTGGAACAACATATACAATAG
AGAGATATTGTAAAATATTATGGAAAGAAAATTGTAGATTGGTAATAAAAGTAATGATAT
CTAAAAAGAAAATAACAAAAACTCAGAAATCCATGGTGAATGCACAACTCTTATTATTGA
GTAAACGATGAGAGAGTGTGTGTGTGTGTGCAGAATGGAACACTATAACTATAACATATG
AAGCTAAAACACTATTTTGCTCTTCTTTCCAAGTTTTCAGTCCACAAGCTTAGCTTTCCG
GTAGCTCTCAACAGAATCCACAAGTGTTTCCTCCAACGGCCGGTAAGTCCACAGGATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCAGCGTTATTA
   
Sequence Name 09-K108066-0022-474-C02C-8409
GenBank Accession LN490212 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K108066-0022-474-C02C-8409
TTCGGACTGAGCCATTTACATTGAATATATCCTGTGGACTTACCGGCCGTTGGAGGAAAC
ACTTGTGGATTCTGTTGAGAGCTACCGGAAAGCTAAGCTTGTGGACTGAAAACTTGGAAA
GAAGAGCAAAATAGTGTTTTAGCTTCATATGTTATAGTTATAGTGTTCCATTCTGCACAC
ACACACACACTCTCTCATCGTTTACTCAATAATAAGAGTTGTGCATTCACCATGGATTTC
TGAGTTTTTGTTATTTTCTTTTTAGATATCATTACTTTTATTACCAATCTACAATTTTCT
TTCCATAATATTTTACAATATCTCTCTATTGTATATGTTGTTCCAATTTCTACCGAAATC
TTACTTTCAAAGGATAAAACACACAAACTTTTATCTCAATTGGTACGTCTTGTTTCTGTA
TAAATTTGTTAATCATTGTAGAAAATAACATAACAAATGTGGATTTAAGCTGAACATAAA
TATTAATGCTTTTTCCGATGAATAAAATTAGTCGAACAAAAGTTGAGATATGGAGCTGAA
GCAGAGATGGTCAAAGTCAAACAA

Last Updated on 06.12.2018 16:11