Sequences from confirmation amplicon


Confirmation sequences for line 475G11 and hit At3g13450 are shown.
Line ID 475G11
Locus At3g13450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4383976
Primers used for PCR 08ht - 8474, show primer details
   
Sequence Name 41-K112586-0022-475-G11C-08ht
GenBank Accession LN504135 [GenBank]
Sequencing primer gene specific primer, 08ht: 5'-TCAAATTTGTGTGATCTTCTCCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K112586-0022-475-G11C-08ht
TTTTAGATTGATTCGTTGGTGGAAAGCGTCTAAATTTTGCGTAAATGGCGGCAGCTCTTG
TACGCAGATTCTGCCGGGGATCATCTTTTCCGGTCTCTGGTCATGGATATAGGATGCTAT
CGACGGTTGAAAATGTATCTGAAAGTGGTAAATCGATGAATCTATACTCTGCTATTAATC
AAGCTCTTCACATCGCTTTAGAGACCGATCCTCGGTGAGCTTTTCTCGCATTTTTCAATC
CTAAAGTCTAAGGTACATTTGAATTGGTTTTTATAATGTATTTGGAGTCACTTGAGTGAA
GGTCTTATGTTTTTGGGGAAGATGTTGGATTTGGTGGTGTCTTCCGATGTACAACAGGAT
TAGCAACAAAAATATATCCTGTTGTACATCAAGATATATTCAATTGTTTCTGGTCTGTAT
CTTCCGATGTAATTCAATTGATGTACAACAGGATATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCACAGCGTTATTA
   
Sequence Name 15-K112585-0022-475-G11C-8409
GenBank Accession LN493041 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K112585-0022-475-G11C-8409
TCGGACTGAGCATTTCATTGAAATATCCTGTTGTACATCAATTGAATTACATCGGAAGAT
ACAGACCAGAAACAATTGAATATATCTTGATGTACAACAGGATATATTTTTGTTGCTAAT
CCTGTTGTACATCGGAAGACACCACCAAATCCAACATCTTCCCCAAAAACATAAGACCTT
CACTCAAGTGACTCCAAATACATTATAAAAACCAATTCAAATGTACCTTAGACTTTAGGA
TTGAAAAATGCGAGAAAAGCTCACCGAGGATCGGTCTCTAAAGCGATGTGAAGAGCTTGA
TTAATAGCAGAGTATAGATTCATCGATTTACCACTTTCAGATACATTTTCAACCGTCGAT
AGCATCCTATATCCATGACCAGAGACCGGAAAAGATGATCCCCGGCAGAATCTGCGTACA
AGAGCTGCCGCCATTTACGCAAAATTTAGACGCTTTCCACCAACGAATCAATCTGAAAAC
TACAGAGGTTTAGCTTAAAGTTCCTCAAGTTTCTTCTGGAGAAGATCAACAAAATTTG

Last Updated on 06.12.2018 16:11