Sequences from confirmation amplicon


Confirmation sequences for line 476D08 and hit At1g75750 are shown.
Line ID 476D08
Locus At1g75750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28441818
Primers used for PCR 27xr - 8474, show primer details
   
Sequence Name 36-K119546-0022-476-D08C-27xr
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 27xr: 5'-CTATGCTACAAAACTTGCGAGTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K119546-0022-476-D08C-27xr
GCCAGATTGTCACAGATTATCGAAAAGACTCCGATGCATAGTGGTATTGCGATCGCTTCG
TAAGCGAAAGGCCGCGAGTTCGATCCTCGCCGGGGGCTTTCAGTTCTTTATTTTTTCCTT
TTTGAAAAATACCAAAATGAATCAACGATGATTTTAACAAAACGAACTTTTCAGTTTTCA
CAAGGGATTTACCAAATCAGTTTACTTCCTCTTGCTAGATTTAAGGAAACTTTACTATCA
TCAATTCATATACGCTAAAGTCATATTTTTGTTTTATTTTCATTTAGACACGAACCTATA
TAAGCTATATGCTATTCATAGGTAATAGGTTCGATGTGTGGACCAAATTTTGGAATAAGG
TATATGGCTGTCAAATAAATATCGATGTTAACAAATATCTTATACCAAAATTCTATCCGT
AATGATCATGATTATTTATGTTCTCGGTCTTCATTTGTCTACTCTATCGTATTAACACAA
ATTATAACGCAAACAAGAATTATTTTTATATGGTCGTGATTAAATAATGGTATAATAAAT
TCAAAATTGTCCGCAAATATATACATCAATATTCTTATACTCAGCTAGTCTAGTAGTTAT
CATCTTGCGTCGATATCAAAATTACTTTATATATCATTCTCGATACTTTTCCAAAACCTC
TTACCATAGAAAAAAAGTATGATAAACAACCACTCAAAGGTAACATAATCTTATTACAAA
TTGAAATAATACAATAATGCACGAATCGATATACAAATATGAAAAAAACAAATTCAAAAC
AAGAAAACTTGCAAGTTAAAACAATAGAACCCTTTATAATACAGTACTCACACTCAC
   
Sequence Name 04-K119545-0022-476-D08C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K119545-0022-476-D08C-8409
ATACATGAAGCCATAAGAAGAAACAAAGCTCTTAATTGCTGCGGATAATGGGACGATGTC
GTTTTGTTAGTATTTACTTTGGCGTATATATGTGGATCGAATAATAAACGAGAACGTACG
TTGTCGTTGTGAGTGTGAGTACTGTATTATTAATGGTTCTATTTGTTTTTACTTGCAAGT
TTTCTTGTTTTGAATTTGTTTTTTTCATATTTGTATATCGATTCGTGCATTATTGTATTA
TTTCAATTTGTAATAAGATTATGTTACCTTTGAGTGGTTGTTTATCATACTTTTTTTCTA
TGGTAAGAGGTTTTGGAAAAGTATCGAGAATGATATATAAAGTAATTTTGATATCGACGC
AAGATGATAACTACTAGACTAGCTGAGTATAAGAATATTGATGTATATATTTGCGGACAA
TTTTGAATTTATTATACCATTATTTAATCACGACCATATAAAAATAATTCTTGTTTGCGT
TATAATTTGTGTTAATACGATAGAGTAGACAAATGAAGACCGAGAACATAAATAATCATG
ATCATTACGGATAGAATTTTGGTATAAGATATTTGTTAACATCGATATTTATTTGACAGC
CATATACCTTATTCCAAAATTTGGTCCACACATCGAACCTATTACCTATGAATAGCATAT
AGCTTATATAGGTTCGTGTCTAAATGAAAATAAAACAAAAATATGACTTTAGCGTATATG
AATTGATGATAGTAAAGGTTTCCTTAAATCTAGCCAGAGGGAATAAACTGATTTGGTAAA
TCCCTTGTGAAAACTGAAAAGTTCGTTTTGTTAAAAT

Last Updated on 06.12.2018 16:11