Sequences from confirmation amplicon


Confirmation sequences for line 478B05 and hit At3g51220 are shown.
Line ID 478B05
Locus At3g51220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19022337
Primers used for PCR 23wj - 8474, show primer details
   
Sequence Name 41-K118761-0022-478-B05C-23wj
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 23wj: 5'-CTGAACACGAGATGAGGATCTCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K118761-0022-478-B05C-23wj
CGGTTCTGCAAGAGCCAGCAGCCAATGGAACCATGGCTTCATCTTCTTCGTCTGCTTCTT
AACCATAACTTGGTTCTTCTCCTCTTTGCCCACAATGACCTTCGTTAACAATGGTGGGTT
AGCAAACTTCACCGAACGCCTCTTCTCTAGACGATCACCATACACTTCATTTCGATCAAA
CCGGTTCATCTTGATCTCGGTTATGTTGTCCGGTTCGTTAACCGTGGATTCCTCTATAAA
CTTGAGGTCATCTTCGACTTGAGGGTGTTCTGGGAAATTTCTGAGTGAGTGATTAAGCTT
TTCTTTAGTGGTTTCAAGCTCTTGTGTAAGGCTTTCGATAAGCTGAGACAAAACCTTGTT
CTCTTCCTCAGCTTTCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTACGCACGATTATT
   
Sequence Name 16-K118760-0022-478-B05C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K118760-0022-478-B05C-8409
CTTTACATTGAAGAAGCTGAGGAAGAGAACAGGTTTTGTCTCAGCTTATCGAAAGCCTTA
CACAAGAGCTTGAAACCACTAAAGAAAAGCTTAATCACTCACTCAGAAATTTCCCAGAAC
ACCCTCAAGTCGAAGATGACCTCAAGTTTATAGAGGAATCCACGGTTAACGAACCGGACA
ACATAACCGAGATCAAGATGAACCGGTTTGATCGAAATGAAGTGTATGGTGATCGTCTAG
AGAAGAGGCGTTCGGTGAAGTTTGCTAACCCACCATTGTTAACGAAGGTCATTGTGGGCA
AAGAGGAGAAGAACCAAGTTATGGTTAAGAAGCAGACGAAGAAGATGAAGCCATTGGTTC
CATTGGCTGCTTGGCTCTTTGCAAGAAACCGGTCTAGTTAAACCACCACTTTAGAGATCC
TCATCTCGTGTTCA

Last Updated on 06.12.2018 16:11