Sequences from confirmation amplicon


Confirmation sequences for line 478B05 and hit At3g51250 are shown.
Line ID 478B05
Locus At3g51250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19028218
Primers used for PCR 26xf - 8474, show primer details
   
Sequence Name 55-K119004-0022-478-B05C-26xf
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 26xf: 5'-AGTTACATCCCCACACCACAGTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K119004-0022-478-B05C-26xf
CATTTAGCAGTACTNTGAGTATAATCCTCGACATTCGGTGCAAGTGTTGTCCAATACGCC
GCACATTGACCTTCCACAACATCCTTTCTCTCACCCTTAAGCTCCTCCGGCGAAGTGTCC
GCCACCACTGAATTTCCCAGCACCTCTTCTCCAGTCTCCTTGGCTTTTTCAGACATTCTC
TGCTCTGTGAAACAACTGTAGTCTCGTAAAACCTGATCAAGCACAAGTAATACATTCTCT
TGCCCTTTCGAAGCAATGGTCAATCCGTAATTAAGAATCTCATCATCCGATTTCGATTTA
CTCTTTTGCCCTTGTTCATCATCTGAATCAGACCCAGATCCTTGACCTTTCTCCTTTGGC
GGATGAATCGAAAAGAAATAGTGAGATCCATCAACCTTAGCTGCAACCTCGTTCTTCGTC
AACGGCCATTGAATCTCATTTCCAACATTGGCTAGAACCGCTACAATGTTTTGCCCCTGG
ATAATTCGAACGATGGTGAAATCACCGCACGCGAGTTCAACGCTGTAGGATTTGTCGATA
AGGTTGAGAATAGCGCCGGGAACACGGATGAGAACCTCCTCCGTCGCCTGAGGAGGAGCA
GATGGTGATTGGTCGTTGGATGCATCTCCGTGGTCTGGGAAGAGATTAGGTCCGAGATTA
TGAGAGGATAGAGAAGGATATAGATAAGGTGAAGAAGCGACGTAAGGATTTGTAGAAGCA
AAAGGATTATCAGGAATGGATTGATNCACTTGGGGATACATCGCCGGTCGTTGCTTGTCG
TCGCCACCGTGAGAAGGATTCATCTTCGTCGTAAAGGATATCACAAATGGCTTCATGTCC
GGGGAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
   
Sequence Name 38-K119003-0022-478-B05C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K119003-0022-478-B05C-8409
ATATCCTTTACGACGAAGATGAATCCTTCTCACGGTGGCGACGACAAGCAACGACCGGCG
ATGTATCCCCAAGTTGATCAATCCATTCCTGATAATCCTTTTGCTTCTACAAATCCTTAC
GTCGCTTCTTCACCTTATCTATATCCTTCTCTATCCTCTCATAATCTCGGACCTAATCTC
TTCCCAGACCACGGAGATGCATCCAACGACCAATCACCATCTGCTCCTCCTCAGGCGACG
GAGGAGGTTCTCATCCGTGTTCCCGGCGCTATTCTCAACCTTATCGACAAATCCTACAGC
GTTGAACTCGCGTGCGGTGATTTCACCATCGTTCGAATTATCCAGGGGCAAAACATTGTA
GCGGTTCTAGCCAATGTTGGAAATGAGATTCAATGGCCGTTGACGAAGAACGAGGTTGCA
GCTAAGGTTGATGGATCTCACTATTTCTTTTCGATTCATCCGCCAAAGGAGAAAGGTCAA
GGATCTGGGTCTGATTCAGATGATGAACAAGGGCAAAAGAGTAAATCGAAATCGGATGAT
GAGATTCTTAATTACGGATTGACCATTGCTTCGAAAGGGCAAGAGAATGTATTACTTGTG
CTTGATCAGGTTTTACGAGACTACAGTTGTTTCACAGAGCAGAGAATGTCTGAAAAAGCC
AAGGAGACTGGAGAAGAGGTGCTGGGAAATTCAGTGGTGGCGGACACTTCGCCGGAGGAG
CTTAAGGGTGAGAGAAAGGATGTTGTGGAAGGTCAATGTGCGGCGTATTGGACAACACTT
GCACCGAATGTCGAGGATTATACTCACAGTACTGCTAAAATGATAGCTTCCGGGTCAGGT
AAGCTGATC

Last Updated on 06.12.2018 16:11