Sequences from confirmation amplicon


Confirmation sequences for line 478C08 and hit At3g57410 are shown.
Line ID 478C08
Locus At3g57410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21249772
Primers used for PCR HV58 - 8474, show primer details
   
Sequence Name 69-K101386-0022-478-C08C-8409
GenBank Accession LN512160 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K101386-0022-478-C08C-8409
CATTTACATTGAATAATCCTGAAACGCAGGATCCAATACTTTTGTTGACCCAGACATTTT
GTATTAGTGGCTAATCTCTTCCTTCAAGAGAAAATACATAAACGACCTGCCAAAAACATA
GAGCAAGCATAAAAAATAAGAAGGTAAGAGATTGTTTTATAAGAAGAAACTGGCGCTTGG
ATAGGAGCCTAAAAAACACTAAAGAGCAACACAAAGGTCTAGCTTTTATAATTGAATTTC
TAAATGGCAATCATATATGAGAGAATATACAGTATTACGTAGAAGCATGTTTGAGCAATA
TATTAACTCTGCAACAATCTGAGTACTCAGTTCTCTCCCACAATTCATTATTATCTGGAA
ATCATTCATAATAATGGTTCCTCAGGGAA
   
Sequence Name 69-K101392-0022-478-C08C-HV58
GenBank Accession LN512161 [GenBank]
Sequencing primer gene specific primer, HV58: 5'-AATTACTTTCCCTGAGGAACCATT-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K101392-0022-478-C08C-HV58
TATATGAATTGTGGGAGAGAATGAGTACTCAGATTGTTGCAGAGTTAATATATTGCTCAA
ACATGCTTCTACGTAATACTGTATATTCTCTCATATATGATTGCCATTTAGAAATTCAAT
TATAAAAGCTAGACCTTTGTGTTGCTCTTTAGTGTTTTTTAGGCTCCTATCCAAGCGCCA
GTTTCTTCTTATAAAACAATCTCTTACCTTCTTATTTTTTATGCTTGCTCTATGTTTTTG
GCAGGTCGTTTATGTATTTTCTCTTGAAGGAAGAGATTAGCCACTAATACAAAATGTCTG
GGTCAACAAAAGTATTGGATCCTGCGTTTCAGGATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGC

Last Updated on 06.12.2018 16:11