Sequences from confirmation amplicon


Confirmation sequences for line 479C07 and hit At3g62060 are shown.
Line ID 479C07
Locus At3g62060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22981163
Primers used for PCR NC67 - 8474, show primer details
   
Sequence Name 36-K101326-0022-479-C07C-8409
GenBank Accession LN502155 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K101326-0022-479-C07C-8409
ATTTACAATTTATAAATTCTTCTTAGTGTACCCTGAATATGTATTATAATTTGTTACAAT
GGCTTTTATGTAGAGTTCGCAACTTTTCTATCGAGGACAACGAATCTGGCAAGTGGCTAT
GGAAGAATTCCTGTCTTTAGGCATGAAGCAAGCAAACCAGGTCTTTGCATTTCATTGAGT
CTATCAGGTTATTGGATTTTGTGCTCTTGTTCATGTTCCGGTTATCGTCTTATCTAACCA
TTTGCAAATAAATATTCATCACAGGCTCTGCTCTCTGGATGTTCAGCTGGAGGATTAGCT
TCTATTTTGCACTGTGATGAGTTCAGGGAACTATTACCTAGTTCCACGAAAGTAAAAT
   
Sequence Name 36-K101332-0022-479-C07C-NC67
GenBank Accession LN502156 [GenBank]
Sequencing primer gene specific primer, NC67: 5'-GCATTTTACTTTCGTGGAACTAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K101332-0022-479-C07C-NC67
AAAAGAAGCTATCCTCCAGCTGAACATCCGAGAGCAGAGCCCGTTATGAATATTTATTTG
CAAATGGTTAGATAAGACGATAACCGGAACATGAACAAGAGCACAAAATCCAATAACCTG
ATAGACTCAATGAAATGCAAAGACCTGGTTTGCTTGCTTCATGCCTAAAGACAGGAATTC
TTCCATAGCCACTTGCCAGATTCGTTGTCCTCGATAGAAAAGTTGCGAACTCTACATAAA
AGCCATTGTAACAAATTATAATACATATTCAGGGTACACTAAGAAGAATTTATTAAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATNTCCGCA

Last Updated on 06.12.2018 16:11