Sequences from confirmation amplicon


Confirmation sequences for line 479D09 and hit At2g38720 are shown.
Line ID 479D09
Locus At2g38720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16189348
Primers used for PCR JV26 - 8474, show primer details
   
Sequence Name 84-K033099-022-479-D09C-8409
GenBank Accession LN514374 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K033099-022-479-D09C-8409
TTACATTGATATATCCTGAGAAAATGAGAGGCTGCTTAAGGTGATAAAAAAATAACACTT
CTGAAGTGTATATATACATATATTATGTGGTTTCTCATGTCTTACCAGTTACCACTGCAC
TTGAGCAATGAGCACAGTAGCATTCAATTAGAAAATGCAGTTTTTATTGGTAGCCTACTA
TTTAACCTGGCACTATTTCTCACGGAAATGAATTCATCAACTCCAAGAAAGCAAACCTTA
GCTTGACGGATTGATCCCTTGATTCTTTTATTTCAGCTACAAGGTTTGGGAAGAAGCATG
CAAGAGCTGTGGAACCTAATGGAAACACCTATGGA
   
Sequence Name 36-K033099-022-479-D09C-JV26
GenBank Accession LN502121 [GenBank]
Sequencing primer gene specific primer, JV26: 5'-TCTTCTCCTCTCATCCATAGGTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K033099-022-479-D09C-JV26
CTTCTTCCAACCTTGTAGCTGAATAAAAGAATCAAGGGATCAATCCGTCAAGCTAAGGTT
TGCTTTCTTGGAGTTGATGAATTCATTTCCGTGAGAAATAGTGCCAGGTTAAATAGTAGG
CTACCAATAAAAACTGCATTTTCTAATTGAATGCTACTGTGCTCATTGCTCAAGTGCAGT
GGTAACTGGTAAGACATGAGAAACCACATAATATATGTATATATACACTTCAGAAGTGTT
ATTTTTTTATCACCTTAAGCAGCCTCTCATGTTTCTCAGGATATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
TAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAA
AGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11